Confirmation of the arginine-finger hypothesis for the GAP-stimulated GTP-hydrolysis reaction of Ras

被引：304

作者：

Ahmadian, MR ^{[1
]}

Stege, P ^{[1
]}

Scheffzek, K ^{[1
]}

Wittinghofer, A ^{[1
]}

机构：

[1] MAX PLANCK INST MOL PHYSIOL,D-44139 DORTMUND,GERMANY

来源：

NATURE STRUCTURAL BIOLOGY | 1997年 / 4卷 / 09期

关键词：

D O I：

10.1038/nsb0997-686

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RasGAPs supply a catalytic residue, termed the arginine finger, into the active site of Ras thereby stabilizing the transition state of the GTPase reaction and increasing the reaction rate by more than one thousand-fold, in good agreement with the structure of the Ras.RasGAP complex.

引用

收藏

页码：686 / 689

页数：4

相关论文

共 44 条

[31] BIOLOGICAL PROPERTIES OF HUMAN C-HA-RAS 1 GENES MUTATED AT CODON-12 [J].

SEEBURG, PH ;

COLBY, WW ;

CAPON, DJ ;

GOEDDEL, DV ;

LEVINSON, AD .

NATURE, 1984, 312 (5989) :71-75

[32]

SKINNER RH, 1991, J BIOL CHEM, V266, P14163

[33] GTPASE MECHANISM OF GPROTEINS FROM THE 1.7-ANGSTROM CRYSTAL-STRUCTURE OF TRANSDUCIN ALPHA-CENTER-DOT-GDP-CENTER-DOT-ALF4(-) [J].

SONDEK, J ;

LAMBRIGHT, DG ;

NOEL, JP ;

HAMM, HE ;

SIGLER, PB .

NATURE, 1994, 372 (6503) :276-279

[34] MOLECULAR-CLONING OF 2-TYPES OF GAP COMPLEMENTARY-DNA FROM HUMAN-PLACENTA [J].

TRAHEY, M ;

WONG, G ;

HALENBECK, R ;

RUBINFELD, B ;

MARTIN, GA ;

LADNER, M ;

LONG, CM ;

CROSIER, WJ ;

WATT, K ;

KOTHS, K ;

MCCORMICK, F .

SCIENCE, 1988, 242 (4886) :1697-1700

[35] A CYTOPLASMIC PROTEIN STIMULATES NORMAL N-RAS P21 GTPASE, BUT DOES NOT AFFECT ONCOGENIC MUTANTS [J].

TRAHEY, M ;

MCCORMICK, F .

SCIENCE, 1987, 238 (4826) :542-545

[36] EXPRESSION OF P21 PROTEINS IN ESCHERICHIA-COLI AND STEREOCHEMISTRY OF THE NUCLEOTIDE-BINDING SITE [J].

TUCKER, J ;

SCZAKIEL, G ;

FEUERSTEIN, J ;

JOHN, J ;

GOODY, RS ;

WITTINGHOFER, A .

EMBO JOURNAL, 1986, 5 (06) :1351-1358

[37] CLONING OF BOVINE GAP AND ITS INTERACTION WITH ONCOGENIC RAS-P21 [J].

VOGEL, US ;

DIXON, RAF ;

SCHABER, MD ;

DIEHL, RE ;

MARSHALL, MS ;

SCOLNICK, EM ;

SIGAL, IS ;

GIBBS, JB .

NATURE, 1988, 335 (6185) :90-93

[38] SIGNAL-TRANSDUCTION PATHWAYS INVOLVING RAS [J].

WIESMULLER, L ;

WITTINGHOFER, F .

CELLULAR SIGNALLING, 1994, 6 (03) :247-267

[39] The interaction of Ras with GTPase-activating proteins [J].

Wittinghofer, A ;

Scheffzek, K ;

Ahmadian, MR .

FEBS LETTERS, 1997, 410 (01) :63-67

[40]

WITTINGHOFER A, 1993, HDB EXPT PHARM, V108, P195

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