Switchable fluorescence of gold nanoclusters for probing the activity of alkaline phosphatase and its application in immunoassay

被引：86

作者：

Hu, Xue-Lian ^{[1
]}

Wu, Xiu-Ming ^{[1
]}

Fang, Xin ^{[1
]}

Li, Zai-Jun ^{[1
]}

Wang, Guang-Li ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Chem & Mat Engn, Minist Educ, Key Lab Food Colloids & Biotechnol, Wuxi 214122, Jiangsu, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2016年 / 77卷

基金：

中国国家自然科学基金;

关键词：

Gold nanoclusters; Alkaline phosphatase; Immunoassay; Fluorescent biosensing; ULTRASENSITIVE DETECTION; QUANTUM DOTS; ASSAY; PROTEIN; ENHANCEMENT; SURFACE; PROBES; NANOPARTICLES; PYROPHOSPHATE; STABILITY;

D O I：

10.1016/j.bios.2015.10.046

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this work, a novel strategy for modulating the fluorescence of gold nanoclusters (Au NCs) is developed. The fluorescence of bovine serum albumin (BSA) protected Au NCs is firstly quenched by KMnO4 and then restored by ascorbic acid (AA) due to the deterioration/restoration of the surface structure. Based on which, a novel "switch-on" fluorescent assay for probing the activity of alkaline phosphatase (ALP) is developed with a detection limit as low as 0.002 U/L. In addition, this testing protocol is also expanded to the detection of the inhibitor of ALP and mouse IgG (as a model), the detection limits are 15 ng/mL for the inhibitor of 2,4-Dichlorophenoxyacetic acid (2,4-DA) and 1.5 pg/mL for mouse IgG. The present method paves a new way to develop convenient, sensitive, and selective metal NCs-based fluorescent "turn-on" probes with promising applications in versatile biosensing. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：666 / 672

页数：7

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