(E)-1-(4-Fluorobenzylidene)-4-(4-ethylphenyl)thiosemicarbazone was synthesized via the reaction of 4-(4-ethylphenyl)thiosemicarbazide and 4-fluorobenzaldehyde. The title compound was characterized by FTIR, H-1 and C-13 NMR, mass spectrometry and elemental analysis techniques. Structural property of the title compound was displayed by the X-ray single crystal diffraction. The title compound crystallized in triclinic space group P (1) over bar with a=0.6494(4) nm, b=0.7971(5) nm, c=1.5492(10) nm, alpha=83.690(11)degrees, beta-84.185(10)degrees, gamma=84.348(11)degrees, molecular formula C16H16FN3S, M-r=301.39, V=0.7868(9) nm(3), Z=2, D-c=1.272 g/cm(3), F(000)=316, mu=0.213 mm(-1), S=1.02, R=0.0513, and omega R[I>2 sigma(I)]=0.1662. The intermolecular interactions in the crystal structure were explained using the Hirshfeld surface and their associated two-dimensional fingerprint plots. The title compound showed C-H center dot center dot center dot S(1-x, -y, -z) and N-H center dot center dot center dot S(1-x, -y, -z) intermolecular interactions, and formed the supramolecular self-assemblies through R-2(2)(12) and R-2(2)(8) ring motifs. Shape index and curvedness were performed to further understand some unique weak interactions, for instance, the weak pi center dot center dot center dot pi stacking contacts in molecular structure with different characteristic regions. Besides, the reduced density gradient(RDG) function provided a real-space function for discussing non-covalent interactions within molecule, such as hydrogen bonds, weak van der Waals interactions and attractive or repulsive effects.