Antifungal Activity of Cyclic Tetrapeptide from Bacillus velezensis CE 100 against Plant Pathogen Colletotrichum gloeosporioides

被引：37

作者：

Choub, Vantha ^{[1
]}

Maung, Chaw Ei Htwe ^{[2
]}

Won, Sang-Jae ^{[1
]}

Moon, Jae-Hyun ^{[1
]}

Kim, Kil Yong ^{[2
]}

Han, Yeon Soo ^{[3
]}

Cho, Jeong-Yong ^{[4
]}

Ahn, Young Sang ^{[1
]}

机构：

[1] Chonnam Natl Univ, Coll Agr & Life Sci, Dept Forest Resources, Gwangju 61186, South Korea

[2] Chonnam Natl Univ, Coll Agr & Life Sci, Inst Environm Friendly Agr, Div Agr & Biol Chem, Gwangju 61186, South Korea

[3] Chonnam Natl Univ, Coll Agr & Life Sci, Inst Environm Friendly Agr, Div Plant Biotechnol, Gwangju 61186, South Korea

[4] Chonnam Natl Univ, Coll Agr & Life Sci, Dept Food Sci & Technol, Gwangju 61186, South Korea

来源：

PATHOGENS | 2021年 / 10卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

antagonistic bacteria; antifungal cyclic tetrapeptide; anthracnose disease; mycelial growth; spore germination; biocontrol agent;

D O I：

10.3390/pathogens10020209

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The aim of this study was to investigate the antifungal activity of a cyclic tetrapeptide from Bacillus velezensis CE 100 against anthracnose-causing fungal pathogen Colletotrichum gloeosporioides. Antifungal compound produced by B. velezensis CE 100 was isolated and purified from ethyl acetate extract of B. velezensis CE 100 culture broth using octadecylsilane column chromatography. The purified compound was identified as cyclo-(prolyl-valyl-alanyl-isoleucyl) based on mass spectrometer and nuclear magnetic resonance analyses. This is the first report of the isolation of a cyclic tetrapeptide from B. velezensis CE 100 culture filtrate. Cyclic tetrapeptide displayed strong antifungal activity at concentration of 1000 mu g/mL against C. gloeosporioides mycelial growth and spore germination. Our results demonstrate that the antifungal cyclic tetrapeptide from B. velezensis CE 100 has potential in bioprotection against anthracnose disease of plants caused by C. gloeosporioides.

引用

页码：1 / 10

页数：10

共 45 条

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