Hydrogen peroxide-induced proline and metabolic pathway of its accumulation in maize seedlings

被引：221

作者：

Yang, Shuang-Long ^{[1
,2
]}

Lan, Shan-Shan ^{[1
,3
]}

Gong, Ming ^{[1
,4
]}

机构：

[1] Yunnan Normal Univ, Coll Life Sci, Kunming 650092, Yunnan, Peoples R China

[2] China Agr Univ, Coll Water Conservancy & Civil Engn, Beijing 100083, Peoples R China

[3] Yunnan Acad Agr, Inst Agr Qual Standard & Testing Tech, Kunming 650223, Peoples R China

[4] Engn Res Ctr Sustainable Dev & Utilizat Biomass E, Minist Educ, Kunming 650092, Peoples R China

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2009年 / 166卷 / 15期

基金：

中国国家自然科学基金;

关键词：

Hydrogen peroxide; Proline biosynthesis and degradation; Zea mays; Delta(1)-pyrroline-5-carboxylate synthetase; DELTA(1)-PYRROLINE-5-CARBOXYLATE SYNTHETASE; FEEDBACK INHIBITION; PHASEOLUS-VULGARIS; OXIDATIVE STRESS; NITRIC-OXIDE; SALT STRESS; PLANTS; BIOSYNTHESIS; DEGRADATION; REMOVAL;

D O I：

10.1016/j.jplph.2009.04.006

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Exogenous H2O2 treatment led to a significant accumulation of proline in coleoptiles and radicles of maize seedlings. It also induced an almost immediate and rapid increase of activity of the key enzymes Delta(1)-pyrroline-5-carboxylate synthetase and glutamate dehydrogenase of the glutamate pathway of proline biosynthesis and an up-regulation of Delta(1)-pyrroline-5-carboxylate synthetase gene expression. Activities of the key enzymes arginase and ornithine ami notransferase of the ornithine pathway of proline biosynthesis increased only after 12h of H2O2 treatment. Furthermore, the H2O2 treatment caused an early decrease of the activity of proline dehydrogenase, a key enzyme of proline degradation. These results indicate that H2O2 might be involved in signal transduction events, leading to proline accumulation in maize seedlings, and that the H2O2-induced proline accumulation is a combined result of the sequential activation of the glutamate and ornithine pathways of proline biosynthesis and the simultaneous inhibition of proline degradation by H2O2. (C) 2009 Elsevier GmbH. All rights reserved.

引用

页码：1694 / 1699

页数：6