Sex chromosome complement determines sex differences in aromatase expression and regulation in the stria terminalis and anterior amygdala of the developing mouse brain

被引：34

作者：

Cisternas, Carla D. ^{[1
,2
]}

Tome, Karina ^{[1
]}

Caeiro, Ximena E. ^{[1
]}

Dadam, Florencia M. ^{[1
]}

Garcia-Segura, Luis M. ^{[3
]}

Cambiasso, Maria J. ^{[1
,2
]}

机构：

[1] INIMEC CONICET UNC, Inst Invest Med Mercedes & Martin Ferreyra, RA-5016 Cordoba, Argentina

[2] Univ Nacl Cordoba, Fac Odontol, Dept Biol Bucal, RA-5000 Cordoba, Argentina

[3] CSIC, Inst Cajal, E-28002 Madrid, Spain

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2015年 / 414卷 / 0C期

关键词：

Sex chromosomes; 17; beta-estradiol; Dihydrotestosterone; Four core genotypes; Sexual differentiation; Aromatase; Androgen receptor; Estrogen receptor; Steroidogenesis; Neurosteroids; MESSENGER-RNA EXPRESSION; RAT-BRAIN; METABOLIZING ENZYMES; ANDROGEN RECEPTOR; FETAL TESTIS; Y-CHROMOSOME; LEYDIG-CELL; NEURONS; 5-ALPHA-REDUCTASE; DIFFERENTIATION;

D O I：

10.1016/j.mce.2015.07.027

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Aromatase, which converts testosterone in estradiol, is involved in the generation of brain sex dimorphisms. Here we used the "four core genotypes" mouse model, in which the effect of gonadal sex and sex chromosome complement is dissociated, to determine if sex chromosomes influence the expression of brain aromatase. The brain of 16 days old XY mouse embryos showed higher aromatase expression in the stria terminalis and the anterior amygdaloid area than the brain of XX embryos, independent of gonadal sex. Furthermore, estradiol or dihydrotestosterone increased aromatase expression in cultures of anterior amygdala neurons derived from XX embryos, but not in those derived from XY embryos. This effect was also independent of gonadal sex. The expression of other steroidogenic molecules, estrogen receptor-a and androgen receptor was not influenced by sex chromosomes. In conclusion, sex chromosomes determine sex dimorphisms in aromatase expression and regulation in the developing mouse brain. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

引用

页码：99 / 110

页数：12

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