Anti-inflammatory effect of austroinulin and 6-O-acetyl-austroinulin from Stevia rebaudiana in lipopolysaccharide-stimulated RAW264.7 macrophages

被引：39

作者：

Cho, Byoung Ok ^{[1
]}

Ryu, Hyung Won ^{[1
]}

So, Yangkang ^{[1
]}

Cho, Jung Keun ^{[1
]}

Woo, Hyun Sim ^{[1
]}

Jin, Chang Hyun ^{[1
]}

Seo, Kwon Il ^{[2
]}

Park, Jong Chun ^{[3
]}

Jeong, Il Yun ^{[1
]}

机构：

[1] Korea Atom Energy Res Inst, Adv Radiat Technol Inst, Jeonbuk 580185, South Korea

[2] Sunchon Natl Univ, Dept Food & Nutr, Jeonnam 540742, South Korea

[3] Seonam Univ, Coll Med, Dept Microbiol, Jeonbuk 590711, South Korea

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2013年 / 62卷

关键词：

Austroinulin; 6-O-acetyl-austroinulin; NO; Pro-inflammatory cytokine; STAT1; IRF3; NF-KAPPA-B; LPS-INDUCED INFLAMMATION; ANTIOXIDANT ACTIVITY; SIGNALING PATHWAY; INOS EXPRESSION; MAPK ACTIVATION; DITERPENES; SUPPRESSES; FLAVONOIDS; STEVIOSIDE;

D O I：

10.1016/j.fct.2013.09.011

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Austroinulin (AI) and 6-O-acetyl-austroinulin (6-OAAI) are natural diterpenoids isolated from Stevia rebaudiana with anti-inflammatory activity. However, the mechanisms underlying their anti-inflammatory effects are not well understood. The purpose of this study was to investigate the effect of AI and 6-OAAI on nitric oxide (NO) production and their molecular mechanism in LPS-stimulated RAW264.7 macrophages. We found that AI and 6-OAAI inhibit the production of NO, iNOS, and pro-inflammatory cytokines (TNF-alpha, IL-6, IL-1 beta, and MCP-1) in LPS-stimulated RAW264.7 macrophages. In these same cells, AI and 6-OAAI also suppressed the phosphorylation of STAT1 and the production of interferon-beta (IFN-beta). Moreover, treatment with AI and 6-OAAI inhibited the activation of interferon regulatory factor-3 (IRF3) and NF-kappa B. Taken together, our results suggest that AI and 6-OAAI inhibit NO production and iNOS expression by blocking the activation of STAT1, IRF3, and NF-kappa B in LPS-stimulated RAW264.7 macrophages. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：638 / 644

页数：7

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