Analysis of porcine adipose tissue transcriptome reveals differences in de novo fatty acid synthesis in pigs with divergent muscle fatty acid composition

被引:83
作者
Corominas, Jordi [1 ,2 ]
Ramayo-Caldas, Yuliaxis [1 ,2 ]
Puig-Oliveras, Anna [1 ,2 ]
Estelle, Jordi [3 ,4 ]
Castello, Anna [1 ,5 ]
Alves, Estefania [6 ]
Pena, Ramona N. [7 ]
Ballester, Maria [1 ,2 ]
Folch, Josep M. [1 ,2 ]
机构
[1] Consorci CSIC IRTA UAB UB, Ctr Recerca Agrigen, Barcelona 08193, Spain
[2] Fac Vet, Dept Ciencia Anim & Aliments, Barcelona 08193, Spain
[3] INRA, UMR Genet Anim & Biol Integrat GABI 1313, Equipe Genet Immunite Sante, F-78352 Jouy En Josas, France
[4] AgroParisTech, UMR GABI 1313, F-78352 Jouy En Josas, France
[5] CEA, DSV iRCM SREIT LREG, F-78352 Jouy En Josas, France
[6] INIA, Dept Mejora Genet Anim, Madrid 28040, Spain
[7] IRTA Lleida, Genet & Millora Anim, Lleida 25198, Spain
来源
BMC GENOMICS | 2013年 / 14卷
关键词
RNA-Seq; Transcriptome; Adipose tissue; Pork; de novo lipogenesis; MEAT QUALITY TRAITS; RNA-SEQ; LIPID-METABOLISM; PEROXISOME PROLIFERATOR; EXPRESSION ANALYSIS; EXTREME PHENOTYPES; GENE-EXPRESSION; LARGE WHITE; INSULIN; PROFILE;
D O I
10.1186/1471-2164-14-843
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: In pigs, adipose tissue is one of the principal organs involved in the regulation of lipid metabolism. It is particularly involved in the overall fatty acid synthesis with consequences in other lipid-target organs such as muscles and the liver. With this in mind, we have used massive, parallel high-throughput sequencing technologies to characterize the porcine adipose tissue transcriptome architecture in six Iberian x Landrace crossbred pigs showing extreme phenotypes for intramuscular fatty acid composition (three per group). Results: High-throughput RNA sequencing was used to generate a whole characterization of adipose tissue (backfat) transcriptome. A total of 4,130 putative unannotated protein-coding sequences were identified in the 20% of reads which mapped in intergenic regions. Furthermore, 36% of the unmapped reads were represented by interspersed repeats, SINEs being the most abundant elements. Differential expression analyses identified 396 candidate genes among divergent animals for intramuscular fatty acid composition. Sixty-two percent of these genes (247/396) presented higher expression in the group of pigs with higher content of intramuscular SFA and MUFA, while the remaining 149 showed higher expression in the group with higher content of PUFA. Pathway analysis related these genes to biological functions and canonical pathways controlling lipid and fatty acid metabolisms. In concordance with the phenotypic classification of animals, the major metabolic pathway differentially modulated between groups was de novo lipogenesis, the group with more PUFA being the one that showed lower expression of lipogenic genes. Conclusions: These results will help in the identification of genetic variants at loci that affect fatty acid composition traits. The implications of these results range from the improvement of porcine meat quality traits to the application of the pig as an animal model of human metabolic diseases.
引用
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页数:14
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