Selenium nanoparticles induce autophagy mediated cell death in human keratinocytes

被引：24
作者：
Kirwale, Shrikant [1 ]
Pooladanda, Venkatesh [1 ]
Thatikonda, Sowjanya [1 ]
Murugappan, Sivasubramanian [2 ]
Khurana, Amit [1 ]
Godugu, Chandraiah [1 ]
机构：
[1] NIPER, Dept Regulatory Toxicol, Hyderabad 500037, Telangana, India
[2] Sri Venkateswara Coll Engn, Dept Chem Engn, Sriperumbudur 602117, Tamil Nadu, India
来源：
NANOMEDICINE | 2019年 / 14卷 / 15期
关键词：
apoptosis; autophagy; cell death; keratinocytes; oxidative stress; SeNPs; REGULATES AUTOPHAGY; BECLIN; ACTIVATION; APOPTOSIS; PROLIFERATION; 5-FLUOROURACIL; CYTOTOXICITY; ANGIOGENESIS; IRRADIATION; MODULATION;
D O I：
10.2217/nnm-2018-0397
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aim: Selenium nanoparticles (SeNPs) may have a potential role in treating dermal disorders due to its wide therapeutic properties, but there is a need to evaluate its toxicity in keratinocytes. The present study evaluated the molecular mechanism and mode of cell death induced by SeNPs on dermal keratinocytes. Materials & methods: SeNPs were synthesized, characterized and studied in human keratinocytes cells. Oxidative stress and mitochondrial membrane depolarization were evaluated by various techniques. Additionally, autophagy mediated apoptotic cell death was evaluated. Results: SeNPs induced oxidative stress and apoptotic cell death in keratinocytes by increasing autophagy through the formation of acidic lysosomes and autophagosomes. Conclusion: Overall, SeNPs induce the oxidative stress and autophagy mediated apoptotic cell death in human keratinocytes cells.
引用
收藏
页码：1991 / 2010
页数：20
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