Molecular tools to understand the bioremediation effect of plants and earthworms on contaminated marine sediments

被引:8
作者
Moreno, Beatriz [1 ]
Canizares, Rosa [1 ]
Macci, Cristina [2 ]
Doni, Serena [2 ]
Masciandaro, Grazia [2 ]
Benitez, Emilio [1 ]
机构
[1] CSIC, EEZ, Environm Protect Dept, E-18008 Granada, Spain
[2] CNR, ISE, I-56124 Pisa, Italy
关键词
Bioremediation; Heavy metals; Hydrocarbons; Marine sediments; Molecular tools; PAH-degrading bacteria; GLUCOSIDASE-ENCODING GENES; SOIL; PHYTOREMEDIATION; DIVERSITY; HYDROCARBONS; REMEDIATION; COMMUNITIES; BACTERIA; COMPOST; SYSTEM;
D O I
10.1016/j.jhazmat.2015.07.019
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A meso-scale pilot plant was set up to test the efficiency of a bioremediation scheme applied to marine sediments contaminated by heavy metals and hydrocarbons. The experiment was implemented for three years in two stages using two remediation agents: plants (Paspalum vaginatum and Tamarix gallica) and earthworms (Eisenia fetida). DNA and RNA-based methodologies were applied to elucidate the dynamics of the bacterial population and were related to improving biological and chemical conditions of the sediments. Bioremediation strategies were successful in removing pollutants from the contaminated sediments and specialization within the bacterial community related to the type of contamination present was detected in the different stages of the process. The highest response of Gram-positive PAH-degraders to the contamination was detected at the beginning and after the first stage of the experiment, corresponding to the uppermost values of degradation. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:398 / 405
页数:8
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