An evaluation of hepatic glucose metabolism at the transcription level for the omnivorous GIFT tilapia, Oreochromis niloticus during postprandial nutritional status transition from anabolism to catabolism

被引:33
作者
Chen, Yong-Jun [1 ,2 ]
Zhang, Ti-Yin [1 ]
Chen, Hai-Yan [1 ]
Lin, Shi-Mei [1 ]
Luo, Li [1 ]
Wang, De-Shou [2 ]
机构
[1] Southwest Univ, Coll Anim Sci & Technol, Key Lab Freshwater Fish Resources & Reprod Dev, Minist Educ, Chongqing, Peoples R China
[2] Southwest Univ, Sch Life Sci, Key Lab Aquat Sci Chongqing, Chongqing, Peoples R China
基金
中国博士后科学基金;
关键词
Glucose metabolism; Transcription; Anabolism; Catabolism; Tilapia; GLUCOSE-6-PHOSPHATASE GENE-EXPRESSION; BREAM SPARUS-AURATA; RAINBOW-TROUT; MOLECULAR PHYSIOLOGY; ENZYME EXPRESSION; LIVER; GLUCOKINASE; FISH; INSULIN; GROWTH;
D O I
10.1016/j.aquaculture.2017.03.009
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
To better understand the regulatory mechanism of glucose metabolism, sub-adult Genetically Improved Farmed Tilapia (GIFT) were sampled at 0, 1, 3, 8 and 24 h after feeding (HAF) after 36 h of food deprivation, and the time course of changes in plasma nutrients and the transcription of a series of key genes involved with hepatic glucose transport and utilization were analyzed. Compared with the fasting state (0 HAF), plasma glucose increased at 1 HAF, peaked at 3 HAF, decreased at 8 HAF and returned to basal level at 24 HAF. Genes such as glucokinase (gck) and liver type of phosphofructokinase (pfkl) were sensitive indicators for hepatic glycolysis, and their transcription was active at 1-8 HAF, especially at 3 HAF. Genes such as glucose-6-phosphatase catalytic subunit a2(g6pca2) and phosphoenolpyruvate carboxykinase 2 (pck2) were useful markers for hepatic gluconeogenesis, and their transcription was inhibited at 1-3 HAF, especially at 3 HAF. Hepatic gluconeogenesis was up-regulated with feeding time from 3 to 8 h, suggesting a nutritional status transition from anabolism to catabolism. The mRNA level of glycogen synthase 1 (gys1) increased at 3-8 HAF and liver glycogen accumulated at 8 HAF, but the principal expressed gys2 was unexpectedly down-regulated after feeding, suggesting that glycogen storage might be controlled by Gys1 rather than Gys2 in the liver of tilapia. In line with the increased plasma triglyceride and cholesterol concentrations, the transcription of hepatic lipogenic genes including acetyl-CoA carboxylase a (acca), ATP citrate lyase a (aclya) and fatty acid synthase (fas) were all up-regulated during 1-8 HAF (especially 3-8 HAF), suggesting that hepatic lipogenesis was an efficient way to dispose excess glucose in tilapia. Hepatic glucose transport was induced since the feeding time was over 8 h, indicating that hepatic glucose production was already transported to extra-hepatic tissues at 8 HAF, which further confirmed the nutritional status transition at this time. Taking the results together, it was suggested that the physiologically specific time point of postprandial nutritional status transition from anabolism to catabolism occurred at about or a little earlier than 8 HAF in tilapia. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:375 / 382
页数:8
相关论文
共 41 条
[31]  
PILKIS SJ, 1992, ANNU REV PHYSIOL, V54, P885, DOI 10.1146/annurev.physiol.54.1.885
[32]   Glucose metabolism in fish: a review [J].
Polakof, Sergio ;
Panserat, Stephane ;
Soengas, Jose L. ;
Moon, Thomas W. .
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY, 2012, 182 (08) :1015-1045
[33]   Dietary carbohydrate-to-protein ratio affects TOR signaling and metabolism-related gene expression in the liver and muscle of rainbow trout after a single meal [J].
Seiliez, Iban ;
Panserat, Stephane ;
Lansard, Marine ;
Polakof, Sergio ;
Plagnes-Juan, Elisabeth ;
Surget, Anne ;
Dias, Karine ;
Larquier, Melanie ;
Kaushik, Sadasivam ;
Skiba-Cassy, Sandrine .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2011, 300 (03) :R733-R743
[34]   Glucokinase and hexokinase expression and activities in rainbow trout tissues: changes with food deprivation and refeeding [J].
Soengas, Jose L. ;
Polakof, Sergio ;
Chen, Xi ;
Sangiao-Alvarellos, Susana ;
Moon, Thomas W. .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2006, 291 (03) :R810-R821
[35]   MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods [J].
Tamura, Koichiro ;
Peterson, Daniel ;
Peterson, Nicholas ;
Stecher, Glen ;
Nei, Masatoshi ;
Kumar, Sudhir .
MOLECULAR BIOLOGY AND EVOLUTION, 2011, 28 (10) :2731-2739
[36]   Effect of Epinephelus coioides, Chanos chanos, and GIFT tilapia in polyculture with Penaeus monodon on the growth of the luminous bacteria Vibrio harveyi [J].
Tendencia, EA ;
Fermin, AC ;
dela Peña, MR ;
Choresca, CH .
AQUACULTURE, 2006, 253 (1-4) :48-56
[37]   GLUT2 is a high affinity glucosamine transporter [J].
Uldry, M ;
Ibberson, M ;
Hosokawa, M ;
Thorens, B .
FEBS LETTERS, 2002, 524 (1-3) :199-203
[38]   New lessons in the regulation of glucose metabolism taught by the glucose 6-phosphatase system [J].
van de Werve, G ;
Lange, A ;
Newgard, C ;
Méchin, MC ;
Li, YZ ;
Berteloot, A .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (06) :1533-1549
[39]   Different modes of activating phosphofructokinase, a key regulatory enzyme of glycolysis, in working vertebrate muscle [J].
Wegener, G ;
Krause, U .
BIOCHEMICAL SOCIETY TRANSACTIONS, 2002, 30 :264-270
[40]   What Is the Metabolic Role of Phosphoenolpyruvate Carboxykinase? [J].
Yang, Jianqi ;
Kalhan, Satish C. ;
Hanson, Richard W. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (40) :27025-27029