siRNA-directed inhibition of HIV-1 infection

被引：627

作者：

Novina, CD ^{[1
]}

Murray, MF

Dykxhoorn, DM

Beresford, PJ

Riess, J

Lee, SK

Collman, RG

Lieberman, J

Shankar, P

Sharp, PA

机构：

[1] Harvard Univ, Sch Med, Ctr Blood Res, Boston, MA 02115 USA

[2] MIT, Ctr Canc Res, Cambridge, MA 02139 USA

[3] MIT, Dept Biol, Cambridge, MA USA

[4] MIT, McGovern Inst Brain Res, Cambridge, MA 02139 USA

[5] Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA

[6] Univ Penn, Sch Med, Philadelphia, PA 19104 USA

来源：

NATURE MEDICINE | 2002年 / 8卷 / 07期

关键词：

D O I：

10.1038/nm725

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA interference silences gene expression through short interfering 21-23-mer double-strand RNA segments that guide mRNA degradation in a sequence-specific fashion. Here we report that siRNAs inhibit virus production by targeting the mRNAs for either the HIV-1 cellular receptor CD4, the viral structural Gag protein or green fluorescence protein substituted for the Nef regulatory protein. siRNAs effectively inhibit pre- and/or post-integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for therapeutic intervention in HIV-1 and other viral infections.

引用

页码：681 / 686

页数：6

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