Anti-Hyperuricemic Effect of Ethyl Acetate Sub-Fractions from Chrysanthemum morifolium Ramat. Dried Flowers on Potassium Oxonate-Induced Hyperuricemic Rats

被引：6

作者：

Ng, Teng Lit ^{[1
]}

Loh, Khye Er ^{[1
]}

Tan, Sheri-Ann ^{[1
]}

Tan, Hui Yin ^{[1
]}

Yue, Chen Son ^{[2
]}

Wee, Sze Ping ^{[1
]}

Tey, Zi Tong ^{[2
]}

机构：

[1] Tunku Abdul Rahman Univ Coll, Fac Appl Sci, Dept Biosci, Jalan Genting Kelang, Kuala Lumpur 53300, Malaysia

[2] Tunku Abdul Rahman Univ Coll, Fac Appl Sci, Dept Phys Sci, Jalan Genting Kelang, Kuala Lumpur 53300, Malaysia

来源：

APPLIED SCIENCES-BASEL | 2022年 / 12卷 / 07期

关键词：

Chrysanthemum morifolium; anti-hyperuricemic; hyperuricemic rat models; xanthine oxidase inhibitor; uric acid reduction; XO gene expression; XANTHINE; IDENTIFICATION; EXPRESSION; EXTRACTS;

D O I：

10.3390/app12073487

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Xanthine oxidase (XO) plays an important role in purine degradation in humans. The study aimed to determine the XO inhibitory potential of Chrysanthemum morifolium dried flower ethyl acetate sub-fractions and its anti-hyperuricemic effect in rat models. Bioassay-guided fractionation based on XO inhibitory assay was employed to obtain bioactive fractions and sub-fractions. In vitro cytotoxicity and cellular antioxidant capacity of the sub-fraction and its mode of XO inhibition were also investigated. The anti-hyperuricemic effect of the bioactive sub-fraction was investigated using rat models via oral consumption, and followed by an XO mRNA gene expression study. The compounds in the bioactive sub-fractions were identified putatively using HPLC-Q-TOF-MS/MS. Ethyl acetate (EtOAc) fraction exhibited the highest XO inhibition among the fractions. It was further fractionated into 15 sub-fractions. F10 exhibited high XO inhibitory activity, cellular pro-proliferative effect, and intracellular antioxidant activity among the sub-fractions tested. This sub-fraction was non-cytotoxic at 0.1-10 mu g/mL, and very effective in lowering serum and urine uric acid level in rat models upon oral consumption. A total of 26 known compounds were identified and seven unknown compounds were detected via HPLC-Q-TOF-MS/MS analysis. The possible mechanisms contributing to the anti-hyperuricemic effect were suggested to be the non-competitive inhibition of XO enzyme, XO gene expression down-regulation, and the enhancement of uric acid excretion.

引用

页数：17

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