We present a full constitutive relation of silicon steel which can describe the anisotropy effect as well. Using a pilot rolling machine, initial silicon strip with thickness of 2.5mm, is rolled into sheet with several thicknesses as reduction ratio increases from 10% to 90%. To examine the effect of anisotropy on the stress-strain behavior, the specimen was cut out from the sheet so that the direction of specimen and sheet is 0 degrees, 30 degrees, 45 degrees, 60 degrees and 90 degrees, respectively. A series of tensile test are then performed with the specimens. The stress-strain curves computed from the proposed constitutive relation are compared with the experimental data. Results show that the predicted curves are in overall in a good agreement with measured ones. The work hardening and unstable softening behaviors of silicon steel during rolling are predicted by the proposed full constitutive relation.