Level of agreement between two commercially available rapid serological tests and the official screening test used to detect Leishmania seropositive dogs in Brazil

被引:14
作者
Dantas-Torres, F. [1 ,2 ]
Sales, K. G. S. [1 ]
da Silva, L. G. [3 ]
Otranto, D. [2 ]
Figueredo, L. A. [1 ]
机构
[1] Fundacao Oswaldo Cruz, Aggeu Magalhaes Inst, Dept Immunol, Recife, PE, Brazil
[2] Univ Bari, Dept Vet Med, Bari, Italy
[3] Ctr Univ Vale Ipojuca UNIFAVIP DeVry, Caruaru, PE, Brazil
关键词
Canine; Leishmaniasis; Rapid diagnostic test; Serology; CANINE VISCERAL LEISHMANIASIS; DIAGNOSIS;
D O I
10.1016/j.tvjl.2018.02.007
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
This study compared the level of agreement between two commercially available rapid serological tests and the official screening test used to detect Leishmania seropositive dogs in Brazil. Ninety-five canine sera from a visceral leishmaniasis endemic area were tested by using the official immunochromatographic test (T1; rK28 antigen) based on dual path platform technology, a rapid ELISA (T2; purified Leishmania antigens) and an immunochromatographic test (T3; rK28 antigen) based on lateral flow. There was substantial agreement (Kappa 0.77; 95% confidence interval, CI, 0.62-0.91; P < 0.001; observed agreement 90.5%) between T1 and T2, and a fair agreement (Kappa 0.26; 95% CI 0.08-0.43; P<0.001; observed agreement 74.7%) between T1 and T3. Sixteen dogs positive at T1 and T2 were negative at T3. T2 may be a reliable alternative to T1, while T3 could lead to an underestimation of the actual number of seropositive dogs. (C) 2018 Elsevier Ltd. All rights reserved.
引用
收藏
页码:102 / 104
页数:3
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