In vitro Evaluation of the Phytochemical and Antioxidant Properties of Bulbine abyssinica Extracts

This study looked to determine the antioxidant properties and phyto-chemical constituents of Bulbine abyssinica leaf using different solvents (aqueous, methanol, ethanol and acetone) against scavenging radicals such as 1,1-diphenyl-2-picrylhydrazyl (ABTS), 2,2-diphenyl-1-piclylhydrazyl (DPPH), Nitric oxide (NO) and total antioxidant capacity (TAC). The polyphenolic constituent's analysis of the leaf extracts (which include flavonoid, flavonols and phenolic content) were likewise evaluated with the utilization of standard phytochemical reaction procedures. The outcome of the phytochemical analyses showed that the leaf extracts had abundance of phenolic compounds, flavonoids and condensed tannins From the result, the value of phenolic constituents found in acetone and aqueous samples gave 11.09 and 7.40 mg tannic acid equivalent/g of extract. Likewise, the values for flavonoid composition of acetone, ethanol and methanol, and aqueous leaf extracts gave 376.05, 320.49 153.52, and 85.42 mg quercetin equivalent per gram of extract, accordingly; moreover, the total phenol leaf extracts gave 24.35, 29.38, 16.31, and 16.00 mg of tannic acid per gram of dry extract sample, respectively. The aqueous, acetone, ethanol and methanol leaf extracts of B. abyssnica gave significant activity (at 0.08 and 0.4 mg/mL) as an antioxidant for ABTS and total anti-oxidant capacity (TAC) radicals when compared with BHT used as a standard drug. The results from this study indicated the antioxidative capabilities of B. abyssnica extracts and the plant may serve as inhibitors (of free radical), which might perform as key antioxidants. (C) 2020 Friends Science Publishers