TaqMan Probe-Based Real-Time PCR Assay for Detection and Discrimination of Class I, II, and III tfdA Genes in Soils Treated with Phenoxy Acid Herbicides

被引：24
作者：
Baelum, Jacob [1 ]
Jacobsen, Carsten S. [1 ,2 ]
机构：
[1] Geol Survey Denmark & Greenland GEUS, Dept Geochem, DK-1350 Copenhagen K, Denmark
[2] Univ Copenhagen, Dept Nat Sci, Fac Life Sci, DK-1871 Frederiksberg, Denmark
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 09期
关键词：
ALCALIGENES-EUTROPHUS JMP134; 2,4-DICHLOROPHENOXYACETIC ACID; 4-CHLORO-2-METHYLPHENOXYACETIC ACID; AGRICULTURAL SOIL; DEGRADATION; MONOOXYGENASE; DIOXYGENASE; METABOLISM; EXPRESSION; BACTERIA;
D O I：
10.1128/AEM.02051-08
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Separate quantification of three classes of tfdA genes was performed using TaqMan quantitative real-time PCR for 13 different soils subsequent to mineralization of three phenoxy acids. Class III tfdA genes were found to be involved in mineralization more often than class I and II tfdA genes.
引用
收藏
页码：2969 / 2972
页数：4
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