TaqMan Probe-Based Real-Time PCR Assay for Detection and Discrimination of Class I, II, and III tfdA Genes in Soils Treated with Phenoxy Acid Herbicides

被引：24

作者：

Baelum, Jacob ^{[1
]}

Jacobsen, Carsten S. ^{[1
,2
]}

机构：

[1] Geol Survey Denmark & Greenland GEUS, Dept Geochem, DK-1350 Copenhagen K, Denmark

[2] Univ Copenhagen, Dept Nat Sci, Fac Life Sci, DK-1871 Frederiksberg, Denmark

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 09期

关键词：

ALCALIGENES-EUTROPHUS JMP134; 2,4-DICHLOROPHENOXYACETIC ACID; 4-CHLORO-2-METHYLPHENOXYACETIC ACID; AGRICULTURAL SOIL; DEGRADATION; MONOOXYGENASE; DIOXYGENASE; METABOLISM; EXPRESSION; BACTERIA;

D O I：

10.1128/AEM.02051-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Separate quantification of three classes of tfdA genes was performed using TaqMan quantitative real-time PCR for 13 different soils subsequent to mineralization of three phenoxy acids. Class III tfdA genes were found to be involved in mineralization more often than class I and II tfdA genes.

引用

页码：2969 / 2972

页数：4

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