Circulating Platelet-Derived Extracellular Vesicles Are a Hallmark of Sars-Cov-2 Infection

被引：97

作者：

Cappellano, Giuseppe ^{[1
,2
]}

Raineri, Davide ^{[1
,2
]}

Rolla, Roberta ^{[1
,3
]}

Giordano, Mara ^{[1
,3
]}

Puricelli, Chiara ^{[3
]}

Vilardo, Beatrice ^{[1
,2
]}

Manfredi, Marcello ^{[2
,4
]}

Cantaluppi, Vincenzo ^{[2
,4
,5
]}

Sainaghi, Pier Paolo ^{[2
,4
,6
]}

Castello, Luigi ^{[4
,7
]}

De Vita, Nello ^{[4
]}

Scotti, Lorenza ^{[4
]}

Vaschetto, Rosanna ^{[4
]}

Dianzani, Umberto ^{[1
,3
]}

Chiocchetti, Annalisa ^{[1
,2
]}

机构：

[1] Univ Piemonte Orientale, Dept Hlth Sci, Interdisciplinary Res Ctr Autoimmune Dis IRCAD, I-28100 Novara, Italy

[2] Univ Piemonte Orientale, Ctr Translat Res Autoimmune & Allerg Dis CAAD, I-28100 Novara, Italy

[3] Maggiore Carita Univ Hosp, Clin Chem Unit, I-28100 Novara, Italy

[4] Univ Piemonte Orientale, Dept Translat Med, I-28100 Novara, Italy

[5] Maggiore Carita Univ Hosp, Nephrol & Kidney Transplantat Unit, I-28100 Novara, Italy

[6] Maggiore Carita Univ Hosp, Immunorheumatol Unit, Div Internal Med, I-28100 Novara, Italy

[7] Maggiore Carita Univ Hosp, Emergency Dept, I-28100 Novara, Italy

来源：

CELLS | 2021年 / 10卷 / 01期

关键词：

circulating biomarker; extracellular vesicles; platelets; COVID-19; Sars-Cov-2; COVID-19; EXOSOMES;

D O I：

10.3390/cells10010085

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Sars-Cov-2 infection causes fever and cough that may rapidly lead to acute respiratory distress syndrome (ARDS). Few biomarkers have been identified but, unfortunately, these are individually poorly specific, and novel biomarkers are needed to better predict patient outcome. The aim of this study was to evaluate the diagnostic performance of circulating platelets (PLT)-derived extracellular vesicles (EVs) as biomarkers for Sars-Cov-2 infection, by setting a rapid and reliable test on unmanipulated blood samples. PLT-EVs were quantified by flow cytometry on two independent cohorts of Sars-CoV-2+ (n = 69), Sars-Cov-2- (n = 62) hospitalized patients, and healthy controls. Diagnostic performance of PLT-EVs was evaluated by receiver operating characteristic (ROC) curve. PLT-EVs count were higher in Sars-Cov-2+ compared to Sars-Cov-2- patients or HC. ROC analysis of the combined cohorts showed an AUC = 0.79 and an optimal cut-off value of 1472 EVs/mu L, with 75% sensitivity and 74% specificity. These data suggest that PLT-EVs might be an interesting biomarker deserving further investigations to test their predictive power.

引用

页码：1 / 10

页数：10

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