Biosynthesis and secretion of recombinant human growth hormone in Pichia pastoris

被引：32

作者：

Ecamilla-Treviño, LL ^{[1
]}

Viader-Salvadó, JM ^{[1
]}

Barrera-Saldaña, HA ^{[1
]}

Guerrero-Olazarán, M ^{[1
]}

机构：

[1] Univ Autonoma Nuevo Leon, Fac Med, Dept Bioquim, Monterrey 64460, NL, Mexico

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 02期

关键词：

alcohol oxidase; gene expression; human growth hormone; Pichia pastoris;

D O I：

10.1023/A:1005675920451

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Mature human growth hormone (hGH) cDNA was cloned by homologous recombination into the yeast Pichia pastoris genome. The hGH gene expression was placed under the control of the methanol-inducible alcohol oxidase 1 (AOX1) gene promoter and the Saccharomyces cerevisiae alpha-factor signal sequence to direct the secretion of recombinant human growth hormone (rhGH) into the growth medium. O-2-limited induction of recombinant yeast strains in shake tubes with 3 ml of culture medium produced up to 11 mg rhGH l(-1), while high cell density cultures using a 2-l bioreactor produced about 49 mg rhGH l(-1) achieving 40% of total protein of the culture medium supernatant.

引用

页码：109 / 114

页数：6

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