Gibberellin production by pure cultures of a new strain of Aspergillus fumigatus

Fungal endophytes produce a variety of favorable metabolites for plant growth and survival, but there is limited information on their gibberellin (GA) production capacity. In the current study, we isolated eight endophytic fungi from the roots of a drought stressed soybean cultivar Hwangkeumkong, and screened them on waito-c rice for plant growth promotion. Seven fungal isolates promoted plant growth, while one inhibited it. The culture filtrate (CF) of fungal isolate HK-5-2 provided the best results for growth promotion and was thus bioassayed on soybean. HK-5-2 CF enhanced plant length, plant fresh and dry weight and endogenous bioactive GA(1) and GA(4) contents of soybean as compared to control. The GA analysis of HK-5-2 CF showed the presence of bioactive GA(3) (8.38 ng/ml), GA(4) (2.16 ng/ml) and GA(7) (1.56 ng/ml) in conjunction with physiologically inactive GA(5), GA(19) and GA(24). Gibberella fujikuroi was used as positive control during this experiment. The fungal isolate HK-5-2 was identified as a new strain of Aspergillus fumigatus through molecular and phylogenetic analysis of 18S and 28S rDNA sequences.