Inactivated vaccine with adjuvants consisting of pattern recognition receptor agonists confers protection against avian influenza viruses in chickens

被引：22

作者：

Tang, Yinghua ^{[1
,2
]}

Lu, Jihu ^{[1
]}

Wu, Peipei ^{[1
]}

Liu, Zhenxing ^{[1
]}

Tian, Zhen ^{[1
]}

Zha, Guofei ^{[1
]}

Chen, Hui ^{[1
]}

Wang, Qiaochu ^{[1
]}

Wang, Qiaoxiu ^{[2
]}

Hou, Fengxiang ^{[1
]}

Kang, Sang-Moo ^{[3
]}

Hou, Jibo ^{[1
]}

机构：

[1] Jiangsu Acad Agr Sci, Natl Res Ctr Engn & Technol Vet Biol, Nanjing 210014, Jiangsu, Peoples R China

[2] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

[3] Georgia State Univ, Ctr Inflammat Immun & Infect, Atlanta, GA 30302 USA

来源：

VETERINARY MICROBIOLOGY | 2014年 / 172卷 / 1-2期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

Avian influenza virus; Inactivated vaccine; Adjuvants; Pattern recognition receptor; Agonist; Toll-like receptor; IMMUNE-RESPONSE; INFECTION; EFFICACY; DISEASES; IMPACT; ROLES; DUCKS; CELLS;

D O I：

10.1016/j.vetmic.2014.05.007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Use of adjuvant containing pathogen pattern recognition receptor agonists is one of the effective strategies to enhance the efficacy of licensed vaccines. In this study, we investigated the efficacy of avian influenza vaccines containing an adjuvant (CVCVA5) which was composed of polyriboinosinic polyribocytidylic, resiquimod, imiquimod, muramyl dipeptide and levomisole. Avian influenza vaccines adjuvanted with CVCVA5 were found to induce significantly higher titers of hemagglutiniton inhibition antibodies (P <= 0.01) than those of commercial vaccines at 2-, 3- and 4-week post vaccination in both specific pathogen free (SPF) chickens and field application. Furthermore, virus shedding was reduced in SPF chickens immunized with H9-CVCVA5 vaccine after H9 subtype heterologous virus challenge. The ratios of both CD3+CD4+ and CD3+CD8+ lymphocytes were slowly elevated in chickens immunized with H9-CVCVA5 vaccine. Lymphocytes adoptive transfer study indicates that CD8+ T lymphocyte subpopulation might have contributed to improved protection against heterologous virus challenge. Results of this study suggest that the adjuvant CVCVA5 was capable of enhancing the potency of existing avian influenza vaccines by increasing humoral and cellular immune response. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：120 / 128

页数：9

共 27 条

[1] A review of the role of CpG oligodeoxynucleotides as toll-like receptor 9 agonists in prophylactic and therapeutic vaccine development in infectious diseases [J].

Gupta, Kaveri ;

Cooper, Curtis .

DRUGS IN R&D, 2008, 9 (03) :137-145

[2] TLR7/8-mediated activation of human NK cells results in accessory cell-dependent IFN-γ production [J].

Hart, OM ;

Athie-Morales, V ;

O'Connor, GM ;

Gardiner, CM .

JOURNAL OF IMMUNOLOGY, 2005, 175 (03) :1636-1642

[3] Synthetic double-stranded RNA poly(I:C) combined with mucosal vaccine protects against influenza virus infection [J].

Ichinohe, T ;

Watanabe, I ;

Ito, S ;

Fujii, H ;

Moriyama, M ;

Tamura, S ;

Takahashi, H ;

Sawa, H ;

Chiba, J ;

Kurata, T ;

Sata, T ;

Hasegawa, H .

JOURNAL OF VIROLOGY, 2005, 79 (05) :2910-2919

[4] Cross-protection against H5N1 influenza virus infection is afforded by intranasal inoculation with seasonal trivalent inactivated influenza vaccine [J].

Ichinohe, Takeshi ;

Tamura, Shin-ichi ;

Kawaguchi, Akira ;

Ninomiya, Ai ;

Imai, Masaki ;

Itamura, Shigeyuki ;

Odagiri, Takato ;

Tashiro, Masato ;

Takahashi, Hidehiro ;

Sawa, Hirofumi ;

Mitchell, William M. ;

Strayer, David R. ;

Carter, William A. ;

Chiba, Joe ;

Kurata, Takeshi ;

Sata, Tetsutaro ;

Hasegawa, Hideki .

JOURNAL OF INFECTIOUS DISEASES, 2007, 196 (09) :1313-1320

[5]

Ichinohe T, 2010, EXPERT REV VACCINES, V9, P1315, DOI [10.1586/erv.10.118, 10.1586/ERV.10.118]

[6] Intracellular NOD-like receptors in host Defense and disease [J].

Kanneganti, Thirumala-Devi ;

Lamkanfi, Mohamed ;

Nunez, Gabriel .

IMMUNITY, 2007, 27 (04) :549-559

[7] The roles of TLRs, RLRs and NLRs in pathogen recognition [J].

Kawai, Taro ;

Akira, Shizuo .

INTERNATIONAL IMMUNOLOGY, 2009, 21 (04) :317-337

[8] Inactivated H9N2 avian influenza virus vaccine with gel-primed and mineral oil-boosted regimen could produce improved immune response in broiler breeders [J].

Lee, D. -H. ;

Kwon, J. -S. ;

Lee, H. -J. ;

Lee, Y. -N. ;

Hur, W. ;

Hong, Y. -H. ;

Lee, J. -B. ;

Park, S. -Y. ;

Choi, I. -S. ;

Song, C. -S. .

POULTRY SCIENCE, 2011, 90 (05) :1020-1022

[9] Long-lasting protection against canine visceral leishmaniasis using the LiESAp-MDP vaccine in endemic areas of France:: Double-blind randomised efficacy field trial [J].

Lemesre, Jean-Loup ;

Holzmuller, Philippe ;

Goncalves, Rachel Bras ;

Bourdoiseau, Gilles ;

Hugnet, Christophe ;

Cavaleyra, Mireille ;

Papierok, Gerard .

VACCINE, 2007, 25 (21) :4223-4234

[10] Experimental challenge of chicken vaccinated with commercially available H5 vaccines reveals loss of protection to some highly pathogenic avian influenza H5N1 strains circulating in Hong Kong/China [J].

Leung, Y. H. Connie ;

Luk, Geraldine ;

Sia, Sin-Fun ;

Wu, Yu-On ;

Ho, Chuk-Kwan ;

Chow, Kai-Chi ;

Tang, Shing-Chun ;

Guan, Yi ;

Peiris, J. S. Malik .

VACCINE, 2013, 31 (35) :3536-3542

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