Expression patterns and role of SDF-1/CXCR4 axis in boar spermatogonial stem cells

被引:9
作者
Park, Hyun Jung [1 ]
Lee, Won Yong [2 ]
Kim, Jin Hoi [1 ]
Park, Chankyu [1 ]
Song, Hyuk [1 ]
机构
[1] Konkuk Univ, Dept Stem Cell & Regenerat Biol, 1 Hwayang Dong, Seoul 05029, South Korea
[2] Korea Natl Coll Agr & Fisheries, Dept Beef & Dairy Sci, Jeonju Si 54874, South Korea
基金
新加坡国家研究基金会;
关键词
Boar; Spermatogonial stem cell; Stromal cell-derive factor 1; C-X-C chemokine receptor type 4; Protein gene product 9.5; Spermatogenesis; IN-VITRO CULTURE; GERM-CELLS; UNDIFFERENTIATED SPERMATOGONIA; DEVELOPMENTAL EXPRESSION; SELF-RENEWAL; CHEMOKINE; RECEPTOR; TESTIS; SDF-1; IDENTIFICATION;
D O I
10.1016/j.theriogenology.2018.03.008
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The signaling of chemokine stromal cell-derived factor (SDF)-1 and its receptor C-X-C motif chemokine receptor 4 (CXCR4) is involved in the cellular proliferation, survival, and migration of various cell types. Although SDF-1/CXCR4 has been implicated in the maintenance of the spermatogonial population during mouse testis development, their expression patterns and functions in boar testis remain unclear. In the present study, the expression pattern of SDF-1 and CXCR4 was determined during pre-pubertal and postpubertal stage boar testes and in vitro cultured porcine spermatogonial stem cells (pSSCs). The role of these proteins in colony formation in cultured pSSCs was also investigated. Interestingly, SDF-1 expression was observed in PGP 9.5-positve spermatogonia in all developing stages of boar testis; however, CXCR4 expression was only detected in spermatogonia from 5-day-old boar testis. In addition, SDF-1 and CXCR4 expression was observed in cultured pSSCs from 5-day-old boar testes, and inhibition of the CXCR4 receptor signaling pathway by AMD3100 significantly decreased the colony formation of pSSCs. These results suggest that SDF-1 and CXCR4 are useful markers for detecting stage-specific spermatogonia in boar testis. Our results reveal the role of the SDF-1/CXCR4 axis in pSSC in vitro culture. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码:221 / 228
页数:8
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