Helicobacter pylori secreted peptidyl prolyl cis, trans-isomerase drives Th17 inflammation in gastric adenocarcinoma

被引:102
作者
Amedei, Amedeo [1 ,2 ]
Munari, Fabio [3 ]
Della Bella, Chiara [1 ]
Niccolai, Elena [1 ]
Benagiano, Marisa [1 ]
Bencini, Lapo [4 ]
Cianchi, Fabio [5 ]
Farsi, Marco [4 ]
Emmi, Giacomo [2 ]
Zanotti, Giuseppe [3 ]
de Bernard, Marina [3 ]
Kundu, Manikuntala [6 ]
D'Elios, Mario Milco [1 ,2 ]
机构
[1] Univ Florence, Dept Internal Med, I-50134 Florence, Italy
[2] AOU Careggi Firenze, I-50134 Florence, Italy
[3] VIMM Padua, I-35129 Padua, Italy
[4] AOU Careggi, Div Gen & Oncol Surg, Florence, Italy
[5] Univ Florence, Dept Med & Surg Crit Care, I-50134 Florence, Italy
[6] Bose Inst, Dept Chem, Kolkata 700009, India
关键词
Gastric cancer; Helicobacter pylori; Pathogenesis; Cytokines; Th17; VEGF; Matrix metalloproteinase; ENDOTHELIAL GROWTH-FACTOR; EPITHELIAL-CELLS; T-CELLS; IMMUNE-RESPONSES; FACTOR-BETA; PROTEIN; MUCOSA; CANCER; IL-17; ANGIOGENESIS;
D O I
10.1007/s11739-012-0867-9
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Helicobacter pylori infection is characterized by an inflammatory infiltrate, consisting mainly of neutrophils and T cells. This study was undertaken to evaluate the type of gastric T cell response elicited by the secreted peptidyl prolyl cis, trans-isomerase of H. pylori (HP0175) in patients with distal gastric adenocarcinoma. The cytokine profile and the effector functions of gastric tumor-infiltrating lymphocytes (TILs) specific for HP0175 was investigated in 20 patients with distal gastric adenocarcinoma and H. pylori infection. The helper function of HP0175-specific TILs for monocyte MMP-2, MMP-9, and VEGF production was also investigated. TILs cells from H. pylori infected patients with distal gastric adenocarcinoma produced Interleukin (IL)-17 and IL-21 in response to HP0175. HP0175-specific TILs showed poor cytolytic activity while expressing helper activity for monocyte MMP-2, MMP-9 and VEGF production. These findings indicate that HP0175 is able to drive gastric Th17 response. Thus, HP0175, by promoting pro-inflammatory low cytotoxic TIL response, matrix degradation and pro-angiogenic pathways, may provide a link between H. pylori and gastric cancer.
引用
收藏
页码:303 / 309
页数:7
相关论文
共 40 条
  • [1] The neutrophil-activating protein of Helicobacter pylori promotes Th1 immune responses
    Amedei, A
    Cappon, A
    Codolo, G
    Cabrelle, A
    Polenghi, A
    Benagiano, M
    Tasca, E
    Azzurri, A
    D'Elios, MM
    Del Prete, G
    de Bernard, M
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 2006, 116 (04) : 1092 - 1101
  • [2] Characterization of tumor antigen peptide-specific T cells isolated from the neoplastic tissue of patients with gastric adenocarcinoma
    Amedei, Amedeo
    Niccolai, Elena
    Della Bella, Chiara
    Cianchi, Fabio
    Trallori, Giacomo
    Benagiano, Marisa
    Bencini, Lapo
    Bernini, Marco
    Farsi, Marco
    Moretti, Renato
    Del Prete, Gianfranco
    D'Elios, Mario Milco
    [J]. CANCER IMMUNOLOGY IMMUNOTHERAPY, 2009, 58 (11) : 1821 - 1832
  • [3] The secreted peptidyl prolyl cis,trans-isomerase HP0175 of Helicobacter pylori induces apoptosis of gastric epithelial cells in a TLR4- and apoptosis signal-regulating kinase 1-dependent manner
    Basak, C
    Pathak, SK
    Bhattacharyya, A
    Pathak, S
    Basu, J
    Kundu, M
    [J]. JOURNAL OF IMMUNOLOGY, 2005, 174 (09) : 5672 - 5680
  • [4] Helicobacter pylori Protein HP0175 Transactivates Epidermal Growth Factor Receptor through TLR4 in Gastric Epithelial Cells
    Basu, Sanchita
    Pathak, Sushil Kumar
    Chatterjee, Gargi
    Pathak, Shresh
    Basu, Joyoti
    Kundu, Manikuntala
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (47) : 32369 - 32376
  • [5] IL-23-mediated regulation of IL-17 production in Helicobacter pylori-infected gastric mucosa
    Caruso, Roberta
    Fina, Daniele
    Paoluzi, Omero Alessandro
    Blanco, Giovanna Del Vecchio
    Stolfi, Carmine
    Rizzo, Angelarnaria
    Caprioli, Flavio
    Sarra, Massimiliano
    Andrei, Fabio
    Fantini, Massimo Claudio
    MacDonald, Thomas T.
    Pallone, Francesco
    Monteleone, Giovanni
    [J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 2008, 38 (02) : 470 - 478
  • [6] IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis
    Caruso, Roberta
    Fina, Daniele
    Peluso, Ilaria
    Fantini, Massimo Claudio
    Tosti, Claudio
    Blanco, Giovanna Del Vecchio
    Paoluzi, Omero Alessandro
    Caprioli, Flavio
    Andrei, Fabio
    Stolfi, Carmine
    Romano, Marco
    Ricci, Vittorio
    MacDonald, Thomas T.
    Pallone, Francesco
    Monteleone, Giovanni
    [J]. JOURNAL OF IMMUNOLOGY, 2007, 178 (09) : 5957 - 5965
  • [7] Borrelia burgdorferi NapA-Driven Th17 Cell Inflammation in Lyme Arthritis
    Codolo, Gaia
    Amedei, Amedeo
    Steere, Allen C.
    Papinutto, Elena
    Cappon, Andrea
    Polenghi, Alessandra
    Benagiano, Marisa
    Paccani, Silvia Rossi
    Sambri, Vittorio
    Del Prete, Gianfranco
    Baldari, Cosima Tatiana
    Zanotti, Giuseppe
    Montecucco, Cesare
    D'Elios, Mario Milco
    de Bernard, Marina
    [J]. ARTHRITIS AND RHEUMATISM, 2008, 58 (11): : 3609 - 3617
  • [8] T cell sensitivity to TGF-β is required for the effector function but not the generation of splenic CD8+ regulatory T cells induced via the injection of antigen into the anterior chamber
    Cone, Robert E.
    Chattopadhyay, Subhasis
    Sharafieh, Roshanak
    Lemire, Yen
    O'Rourke, James
    Flavell, Richard A.
    Clark, Robert B.
    [J]. INTERNATIONAL IMMUNOLOGY, 2009, 21 (05) : 567 - 574
  • [9] Inflammation, Immunity, and Vaccines for Helicobacter pylori
    D'Elios, Mario M.
    Andersen, Leif P.
    [J]. HELICOBACTER, 2009, 14 : 21 - 28
  • [10] Helicobacter pylori, T cells and cytokines:: the "dangerous liaisons"
    D'Elios, MM
    Amedei, A
    Benagiano, M
    Azzurri, A
    Del Prete, G
    [J]. FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2005, 44 (02): : 113 - 119