Protein-nucleic acid interactions and the expanding role of mass spectrometry

被引：30

作者：

Hanson, CL ^{[1
]}

Robinson, CV ^{[1
]}

机构：

[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2004年 / 279卷 / 24期

关键词：

D O I：

10.1074/jbc.R300037200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mass spectrometry methods are being developed that enable detection of protein interactions with nucleic acids. By mass measuring complexes a direct determination of the stoichiometry of protein-nucleic acid interactions is revealed. For more complex assemblies, using a different approach it is possible to gain information about subcomplexes and even the spatial arrangement of proteins in macromolecular machines. To illustrate these different approaches we review progress and problems encountered in evaluating complexes from bimolecular interactions through to macromolecular machines such as ribosomes.

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页码：24907 / 24910

页数：4

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