Expression of the folate receptor genes FOLR1 and FOLR3 differentiates ovarian carcinoma from breast carcinoma and malignant mesothelioma in serous effusions

被引：61
作者：
Yuan, Yuan [2 ]
Nymoen, Dag Andre
Dong, Hiep Phuc
Bjorang, Ola
Shih, Ie-Ming [6 ,7 ,8 ]
Low, Philip S. [4 ]
Trope, Claes G. [1 ,5 ]
Davidson, Ben [3 ,5 ]
机构：
[1] Rikshosp Univ Hosp, Norwegian Radium Hosp, Div Obstet & Gynecol, N-0310 Oslo, Norway
[2] Rikshosp Univ Hosp, Natl Resource Ctr Womens Hlth, N-0316 Oslo, Norway
[3] Rikshosp Univ Hosp, Norwegian Radium Hosp, Dept Pathol, Div Pathol, N-0310 Oslo, Norway
[4] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
[5] Univ Oslo, Fac Med, Fac Div Radiumhosp, N-0316 Oslo, Norway
[6] Johns Hopkins Univ, Inst Med, Dept Pathol, Baltimore, MD USA
[7] Johns Hopkins Univ, Inst Med, Dept Gynecol, Baltimore, MD USA
[8] Johns Hopkins Univ, Inst Med, Dept Oncol, Baltimore, MD USA
来源：
HUMAN PATHOLOGY | 2009年 / 40卷 / 10期
关键词：
Folate receptors; Serous effusions; Ovarian carcinoma; Breast carcinoma; Malignant mesothelioma; Survival; BINDING-PROTEIN; NORMAL-TISSUES; GROWTH-FACTOR; CANCER-CELLS; SURVIVAL; CYTOLOGY; ALPHA; OVEREXPRESSION; DIAGNOSIS; MARKERS;
D O I：
10.1016/j.humpath.2009.02.013
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
The objective of this study was to analyze the diagnostic and clinical role of the folate receptor-or. (FOLR1) and folate receptor-gamma (FOLR3) genes in effusion cytology. Expression of the FOLR1 protein product, FR-alpha, was additionally studied. Ninety-one effusions (71 ovarian carcinomas, 10 breast carcinomas, 10 malignant mesotheliomas) were assayed for FOLR1 and FOLR3 gene expression using quantitative polymerase chain reaction. FR-alpha expression was analyzed using flow cytometry. Ovarian carcinoma expression levels were analyzed for association with clinicopathologic parameters and survival. Quantitative polymerase chain reaction analysis showed significantly higher FOLR1 and FOLR3 mRNA levels in ovarian carcinomas compared with both breast carcinomas and mesotheliomas (P < .001). FOLR1 and FOLR3 mRNA levels were directly interrelated in ovarian carcinoma (P<.001). FR-a protein levels were similarly higher in ovarian carcinoma compared with the 2 other cancer types (P < .001). FOLR1 and FOLR3 mRNA and FR-alpha protein expression in ovarian carcinoma effusions showed no association with clinical parameters or survival. Our data suggest that folate receptor levels effectively differentiate ovarian carcinoma from other cancers affecting the serosal cavities and that folate receptor genes are coexpressed in this tumor. The high expression of folate receptors in ovarian carcinoma supports their validity as molecular therapeutic targets in this disease. (c) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：1453 / 1460
页数：8
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