Probing Exchange Pathways in One-Dimensional Aggregates with Super-Resolution Microscopy

被引：234
作者：
Albertazzi, Lorenzo [1 ,2 ]
van der Zwaag, Daan [1 ,3 ]
Leenders, Christianus M. A. [1 ,3 ]
Fitzner, Robert [4 ]
van der Hofstad, Remco W. [1 ,4 ]
Meijer, E. W. [1 ,2 ,3 ]
机构：
[1] Eindhoven Univ Technol, Inst Complex Mol Syst, NL-5600 MD Eindhoven, Netherlands
[2] Eindhoven Univ Technol, Lab Chem Biol, NL-5600 MD Eindhoven, Netherlands
[3] Eindhoven Univ Technol, Lab Macromol & Organ Chem, NL-5600 MD Eindhoven, Netherlands
[4] Eindhoven Univ Technol, Dept Math & Comp Sci, NL-5600 MD Eindhoven, Netherlands
来源：
SCIENCE | 2014年 / 344卷 / 6183期
基金：
欧洲研究理事会;
关键词：
SUPRAMOLECULAR POLYMERIZATION; POLYMERS; KINETICS; IMAGES;
D O I：
10.1126/science.1250945
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Supramolecular fibers are prominent structures in biology and chemistry. A quantitative understanding of molecular exchange pathways in these one-dimensional aggregates was obtained by a combination of super-resolution stochastic optical reconstruction microscopy and stochastic simulation. The potential of this methodology is demonstrated with a set of well-defined synthetic building blocks that self-assemble into supramolecular fibrils. Previous ensemble measurements hid all molecular phenomena underpinning monomer exchange, but the molecular pathway determined from single-aggregate studies revealed unexpected homogeneous exchange along the polymer backbone. These results pave the way for experimental investigation of the structure and exchange pathways of synthetic and natural supramolecular fibers.
引用
收藏
页码：491 / 495
页数：5
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