Evaluation of the Xpert MTB/RIF Assay in Gastric Lavage Aspirates for Diagnosis of Smear-negative Childhood Pulmonary Tuberculosis

被引:43
|
作者
Pang, Yu [1 ,2 ]
Wang, Yufeng [2 ]
Zhao, Shunying [1 ]
Liu, Jinrong [1 ]
Zhao, Yanlin [2 ]
Li, Huimin [1 ]
机构
[1] Capital Med Univ, Beijing Childrens Hosp, Beijing 100045, Peoples R China
[2] Chinese Ctr Dis Control & Prevent, Natl Ctr TB Control & Prevent, Beijing, Peoples R China
关键词
tuberculosis; Xpert MTB/RIF; gastric lavage aspirate; MYCOBACTERIUM-TUBERCULOSIS; CHILDREN; ACCURACY; PROGRESS; AFRICA;
D O I
10.1097/INF.0000000000000403
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background: The diagnosis of pulmonary tuberculosis (TB) in children presents problems worldwide. The Xpert MTB/RIF assay serves as a useful alternative for the rapid diagnosis of childhood TB, with various clinical specimens. Methods: In this study, we evaluated the Xpert for diagnosis of smear-negative childhood pulmonary TB using gastric lavage aspirates (GLA). A total of 211 eligible children with suspected TB were consecutively recruited and had GLA obtained for analysis between October 2012 and October 2013 in Beijing Children's Hospital. Results: The percentages of TB children in < 1 year [Odds ratio (95% confidence interval): 4.66 (1.81-11.97)], > 5 years group [2.59 (1.30-5.16)] and of living in a rural region [3.78 (2.02-7.08)] were significantly higher than in control groups. The sensitivity in detecting children with a clinical diagnosis of TB for MGIT and Xpert was 12.1% (95% confidence interval: 9.3-14.9%) and 48.6% (95% confidence interval: 44.4-52.8%), respectively. The statistical analysis revealed that GeneXpert showed significantly better sensitivity than MGIT technology (P < 0.001). Conclusions: We conclude that Xpert MTB/RIF assay is an excellent tool for the diagnosis of smear-negative childhood with GLA samples. The high proportion of very low mycobacterial load in the GLA samples from smear-negative TB cases may increase the frequency for obtaining indeterminate RIF resistance results by Xpert. In addition, the age < 1 year, > 5 years and living in a rural region are all high risk factors for childhood TB cases in China.
引用
收藏
页码:1047 / 1051
页数:5
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