Colorimetric biosensor for the assay of paraoxon in environmental water samples based on the iodine-starch color reaction

被引：43

作者：

Guo, Liangqia ^{[1
]}

Li, Zhihong ^{[1
]}

Chen, Huan ^{[1
]}

Wu, Yarong ^{[1
]}

Chen, Ling ^{[1
]}

Song, Zhiping ^{[1
]}

Lin, Tianran ^{[1
]}

机构：

[1] Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Key Lab Anal & Detect Food Safety, Minist Educ,Coll Chem, Fuzhou 350116, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2017年 / 967卷

关键词：

Organophosphorus pesticides; Acetylcholinesterase; Colorimetric method; Biosensor; Paraoxon; ORGANOPHOSPHORUS PESTICIDES; LIQUID-CHROMATOGRAPHY; CARBON NANOTUBES; ACETYLCHOLINESTERASE; CHOLINESTERASE; CHLORPYRIFOS; FRUIT;

D O I：

10.1016/j.aca.2017.02.028

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, a new colorimetric biosensor for the assay of paraoxon was developed via the conventional iodine-starch color reaction and multi-enzyme cascade catalytic reactions. In the presence of acetylcholine chloride, acetylcholinesterase (AChE) and choline oxidase (ChO) catalyzed the formation of H2O2, which then activated horseradish peroxidase (HRP) to catalyze the oxidation of KI to produce an iodinestarch color reaction. Upon exposure to paraoxon, the catalytic activity of AChE was inhibited and less H2O2 generated, resulting in a decrease in the production of I-2 and a drop in the intensity of solution color. This colorimetric biosensor showed high sensitivity for the assay of paraoxon with a limit of detection 4.7 ppb and was applied for the assay of paraoxon in spiked real samples. By employing the conventional iodine-starch color reaction, this biosensor has the potential of on-site assay of OPs residues in environmental samples. (C) 2017 Elsevier B. V. All rights reserved.

引用

页码：59 / 63

页数：5

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