Phosphorylation of G3BP1-S149 does not influence stress granule assembly

被引：39

作者：

Panas, Marc D. ^{[1
,2
,3
]}

Kedersha, Nancy ^{[1
,2
]}

Schulte, Tim ^{[4
]}

Branca, Rui M. ^{[5
]}

Ivanov, Pavel ^{[1
,2
]}

Anderson, Paul ^{[1
,2
]}

机构：

[1] Harvard Med Sch, Div Rheumatol Immunol & Allergy, Boston, MA 02115 USA

[2] Brigham & Womens Hosp, 75 Francis St, Boston, MA 02115 USA

[3] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden

[4] Karolinska Inst, Dept Med Solna, Sci Life Lab, Stockholm, Sweden

[5] Karolinska Inst, Dept Oncol Pathol, Sci Life Lab, Clin Prote Mass Spectrometry, Stockholm, Sweden

来源：

JOURNAL OF CELL BIOLOGY | 2019年 / 218卷 / 07期

基金：

美国国家卫生研究院;

关键词：

MEDIATE;

D O I：

10.1083/jcb.201801214

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Tourriere et al. (2013. J. Cell Biol. https://doi.org/10.1083/jcb.200212128) reported that G3BP1-S149 dephosphorylation promotes stress granule formation. We show that constructs used to establish this conclusion contain additional mutations causing these phenotypes, and that S149 phosphorylation status does not change upon stress.

引用

页码：2425 / 2432

页数：8

共 17 条

[1] PHENIX:: building new software for automated crystallographic structure determination [J].

Adams, PD ;

Grosse-Kunstleve, RW ;

Hung, LW ;

Ioerger, TR ;

McCoy, AJ ;

Moriarty, NW ;

Read, RJ ;

Sacchettini, JC ;

Sauter, NK ;

Terwilliger, TC .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2002, 58 :1948-1954

[2] MolProbity: all-atom contacts and structure validation for proteins and nucleic acids [J].

Davis, Ian W. ;

Leaver-Fay, Andrew ;

Chen, Vincent B. ;

Block, Jeremy N. ;

Kapral, Gary J. ;

Wang, Xueyi ;

Murray, Laura W. ;

Arendall, W. Bryan, III ;

Snoeyink, Jack ;

Richardson, Jane S. ;

Richardson, David C. .

NUCLEIC ACIDS RESEARCH, 2007, 35 :W375-W383

[3] The backrub motion: How protein backbone shrugs when a sidechain dances [J].

Davis, IW ;

Arendall, WB ;

Richardson, DC ;

Richardson, JS .

STRUCTURE, 2006, 14 (02) :265-274

[4] Coot:: model-building tools for molecular graphics [J].

Emsley, P ;

Cowtan, K .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 :2126-2132

[5] Ultrasensitive proteome analysis using paramagnetic bead technology [J].

Hughes, Christopher S. ;

Foehr, Sophia ;

Garfield, David A. ;

Furlong, Eileen E. ;

Steinmetz, Lars M. ;

Krijgsveld, Jeroen .

MOLECULAR SYSTEMS BIOLOGY, 2014, 10 (10)

[6] Dynamic shuttling of TIA-1 accompanies the recruitment of mRNA to mammalian stress granules [J].

Kedersha, N ;

Cho, MR ;

Li, W ;

Yacono, PW ;

Chen, S ;

Gilks, N ;

Golan, DE ;

Anderson, P .

JOURNAL OF CELL BIOLOGY, 2000, 151 (06) :1257-1268

[7] Mammalian stress granules and processing bodies [J].

Kedersha, Nancy ;

Anderson, Paul .

TRANSLATION INITIATION: CELL BIOLOGY, HIGH-THROUGHPUT METHODS, AND CHEMICAL-BASED APPROACHES, 2007, 431 :61-+

[8] G3BP-Caprin 1-USP10 complexes mediate stress granule condensation and associate with 40S subunits [J].

Kedersha, Nancy ;

Panas, Marc D. ;

Achorn, Christopher A. ;

Lyons, Shawn ;

Tisdale, Sarah ;

Hickman, Tyler ;

Thomas, Marshall ;

Lieberman, Judy ;

McInerney, Gerald M. ;

Ivanov, Pavel ;

Anderson, Paul .

JOURNAL OF CELL BIOLOGY, 2016, 212 (07) :845-860

[9] Stress granules and cell signaling: more than just a passing phase? [J].

Kedersha, Nancy ;

Ivanov, Pavel ;

Anderson, Paul .

TRENDS IN BIOCHEMICAL SCIENCES, 2013, 38 (10) :494-506

[10] Modulation of protein properties in living cells using nanobodies [J].

Kirchhofer, Axel ;

Helma, Jonas ;

Schmidthals, Katrin ;

Frauer, Carina ;

Cui, Sheng ;

Karcher, Annette ;

Pellis, Mireille ;

Muyldermans, Serge ;

Casas-Delucchi, Corella S. ;

Cardoso, M. Cristina ;

Leonhardt, Heinrich ;

Hopfner, Karl-Peter ;

Rothbauer, Ulrich .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2010, 17 (01) :133-U162

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