O-Methylisourea Can React with the α-Amino Group of Lysine: Implications for the Analysis of Reactive Lysine

被引：3

作者：

Hulshof, Tetske G. ^{[1
,2
]}

Rutherfurd, Shane M. ^{[3
]}

Sforza, Stefano ^{[4
,5
]}

Bikker, Paul ^{[1
]}

van der Poel, Antonius F. B. ^{[2
]}

Hendriks, Wouter H. ^{[2
]}

机构：

[1] Wageningen Univ & Res, Wageningen Livestock Res, POB 338, NL-6700 AH Wageningen, Netherlands

[2] Wageningen Univ & Res, Anim Nutr Grp, POB 338, NL-6700 AH Wageningen, Netherlands

[3] Massey Univ, Riddet Inst, Private Bag 11222, Palmerston North, New Zealand

[4] Wageningen Univ & Res, Lab Food Chem, POB 17, NL-6700 AH Wageningen, Netherlands

[5] Univ Parma, Dept Food Sci, Parco Area Sci 59-A, I-43124 Parma, Italy

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2017年 / 65卷 / 04期

关键词：

guanidination reaction; reaction conditions; specificity; reactive lysine; DESORPTION/IONIZATION MASS-SPECTROMETRY; AVAILABLE LYSINE; INTESTINAL-ABSORPTION; ACID DIGESTIBILITY; PROTEIN HYDROLYSIS; MAILLARD REACTION; SYNTHETIC LYSINE; GROWING PIGS; GUANIDINATION; PRODUCTS;

D O I：

10.1021/acs.jafc.6b03096

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The specificity of O-methylisourea (OMIU) to bind to the epsilon-amino group of Lys, an important supposition for the OMIU-reactive Lys analysis of foods, feeds, ingredients, and digesta, was investigated. Crystalline L-Lys incubated under standard conditions with OMIU resulted in low homoarginine recoveries. The reaction of OMIU with the alpha-amino group of Lys was confirmed by MS analysis, with double derivatized Lys being identified. None of the changes in reaction conditions (OMIU pH, OMIU to Lys ratio, and reaction time) with crystalline L-Lys resulted in 100% recovery of homoarginine. The average free Lys content in ileal digesta of growing pigs and broilers was found to be 13% of total Lys, which could result in a significant underestimation of the reactive Lys content. The reaction of OMIU with alpha-amino groups may necessitate analysis of free Lys to accurately quantify reactive lysine in samples containing a large proportion of Lys with a free alpha-amino group.

引用

页码：964 / 972

页数：9

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