DISTRIBUTION OF MACROLIDE-RESISTANT GENES AMONG ISOLATES OF MACROLIDE-RESISTANT STREPTOCOCCUS PYOGENES AND STREPTOCOCCUS PNEUMONIAE IN SERBIA

被引：6

作者：

Gajic, Ina ^{[1
]}

Mijac, Vera ^{[1
]}

Opavski, Natasa ^{[1
]}

Stanojevic, Maja ^{[1
]}

Lazarevic, Ivana ^{[1
]}

Smitran, Aleksandra ^{[2
]}

Hadnadev, Mirjana ^{[3
]}

Ranin, L. ^{[1
]}

机构：

[1] Univ Belgrade, Fac Med, Inst Microbiol & Immunol, Belgrade 11000, Serbia

[2] Univ Banja Luka, Fac Med, Dept Microbiol & Immunol, Banja Luka 78000, Republic Of Srp, Bosnia & Herceg

[3] Inst Pulm Dis Vojvodina, Sremska Kamenica 21204, Serbia

来源：

ARCHIVES OF BIOLOGICAL SCIENCES | 2014年 / 66卷 / 01期

关键词：

Streptococcus pyogenes; Streptococcus pneumoniae; macrolide resistance; MECHANISMS; TELITHROMYCIN; SURVEILLANCE; CLINDAMYCIN; PHENOTYPES; MEF(A); SPAIN;

D O I：

10.2298/ABS1401093G

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Macrolide resistance in Streptococcus pneumoniae and in group A streptococci (GAS) is a significant problem worldwide. In Serbia, data on the mechanisms of resistance and the corresponding resistance genes in streptococci are largely lacking. Therefore, we analyzed the distribution of macrolide resistance phenotypes and genotypes in 44 macrolide-resistant GAS (MRGAS) and 50 macrolide-resistant S. pneumoniae (MRSP) isolates collected in the same period. The double disk diffusion test and PCR were used to analyze resistance phenotypes and resistance genes, respectively. Among MRSP, the MLSB phenotype dominated, whereas the M phenotype was the most prevalent among MRGAS isolates. Consequently, in MRSP, the ermB gene was the most common (n=40, 80%), followed by the mefA gene (n=7,14%). In MRGAS strains, mefA dominated (n=27, 61%), followed by ermA (n=15, 33%) and ermB (n=3, 7%). In 3 MRSP isolates no resistance genes were detected, while one MRGAS strain with iMLS(B) phenotype harbored both ermA and mefA genes.

引用

页码：93 / 98

页数：6

共 22 条

[1]

[Anonymous], 2021, Breakpoint Tables for Interpretation of MICs and Zone Diameters

[2] mef(A) is the predominant macrolide resistance determinant in Streptococcus pneumoniae and Streptococcus pyogenes in Germany [J].

Bley, Christine ;

van der Linden, Mark ;

Reinert, Ralf Rene .

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2011, 37 (05) :425-431

[3] The global burden of group A streptococcal diseases [J].

Carapetis, JR ;

Steer, AC ;

Mulholland, EK ;

Weber, M .

LANCET INFECTIOUS DISEASES, 2005, 5 (11) :685-694

[4] Decline in macrolide-resistant Streptococcus pyogenes isolates from French children [J].

d'Humieres, Camille ;

Cohen, Robert ;

Levy, Corinne ;

Bidet, Philippe ;

Thollot, Franck ;

Wollner, Alain ;

Bingen, Edouard .

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2012, 302 (7-8) :300-303

[5] Detection of macrolide resistance mechanisms in Streptococcus pneumonide and Streptococcus pyogenes using a multiplex rapid cycle PCR with microwell-format probe hybridization [J].

Farrell, DJ ;

Morrissey, I ;

Bakker, S ;

Felmingham, D .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2001, 48 (04) :541-544

[6] Mechanisms of macrolide resistance in clinical pneumococcal isolates in France [J].

Fitoussi, F ;

Doit, C ;

Geslin, P ;

Brahimi, N ;

Bingen, E .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (02) :636-638

[7] Antimicrobial susceptibility patterns of β-hemolytic and viridans group streptococci:: report from the SENTRY Antimicrobial Surveillance Program (1997-2000) [J].

Gordon, KA ;

Beach, ML ;

Biedenbach, DJ ;

Jones, RN ;

Rhomberg, PR ;

Mutnick, AH .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2002, 43 (02) :157-162

[8]

Green M.D., 2003, J ANTIMICROB CHEMOTH, V52, P229

[9] Increase in Pneumococcus Macrolide Resistance, United States [J].

Jenkins, Stephen G. ;

Farrell, David J. .

EMERGING INFECTIOUS DISEASES, 2009, 15 (08) :1260-1264

[10] Mechanisms of resistance to macrolides and lincosamides: Nature of the resistance elements and their clinical implications [J].

Leclercq, R .

CLINICAL INFECTIOUS DISEASES, 2002, 34 (04) :482-492

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