Measurement of concentration and viability of brook trout (Salvelinus fontinalis) spermatozoa using computer-aided fluorescent microscopy

被引：37
作者：
Nynca, Joanna [1 ]
Ciereszko, Andrzej [1 ]
机构：
[1] Polish Acad Sci, Inst Anim Reprod & Food Res, PL-10747 Olsztyn, Poland
来源：
AQUACULTURE | 2009年 / 292卷 / 3-4期
关键词：
Brook trout; Sperm; Concentration; Viability; SPERM VIABILITY; RAINBOW-TROUT; CRYOPRESERVATION; WHITEFISH; QUALITY; FISH;
D O I：
10.1016/j.aquaculture.2009.04.020
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The usefulness of computer-aided fluorescent microscopy for measuring the concentration and viability of brook trout spermatozoa was tested with the NucleoCounter SP-100 system (Chemometec, Denmark). Sperm concentration measured with the new method significantly correlated with results obtained with the use of the photometric method (r(2) = 0.96; P < 0.0001; n = 23). A dose-dependent relationship was found between the percentage of killed spermatozoa and viability. Our data suggest that using computer-aided fluorescent microscopy with the employment of NucleoCounter SP-100 offers an accurate and fast measurement of sperm concentration and viability of fish semen. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：256 / 258
页数：3
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