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Ligustilide ameliorates neuroinflammation and brain injury in focal cerebral ischemia/reperfusion rats: involvement of inhibition of TLR4/peroxiredoxin 6 signaling
被引：103
作者：
Kuang, Xi
[1
]
Wang, Liang-Fen
[1
]
Yu, Lu
[1
]
Li, Yong-Jie
[1
]
Wang, Yan-Nan
[1
]
He, Qian
[1
]
Chen, Chu
[1
]
Du, Jun-Rong
[1
]
机构：
[1] Sichuan Univ, West China Sch Pharm, Dept Pharmacol, Key Lab Drug Targeting & Drug Delivery Syst, Chengdu 610041, Peoples R China
基金：
中国国家自然科学基金;
关键词：
lschemic stroke;
Neuroinflammation;
Immunomodulation;
TLR4;
signaling;
Peroxiredoxin;
6;
NF-kappa B;
STAT3;
Ligustilide;
Free radicals;
TOLL-LIKE RECEPTORS;
ACUTE ISCHEMIC-STROKE;
T-LYMPHOCYTES;
INFLAMMATION;
EXPRESSION;
DAMAGE;
TOLL-LIKE-RECEPTOR-4;
NEUROPROTECTION;
REPERFUSION;
PROTECTS;
D O I：
10.1016/j.freeradbiomed.2014.03.028
中图分类号：
Q5 [生物化学];
Q7 [分子生物学];
学科分类号：
071010 ;
081704 ;
摘要：
Blocking TLR4/peroxiredoxin (Prx6) signaling is proposed to be a novel therapeutic strategy for ischemic stroke because extracellular Prx6 released from ischemic cells may act as an endogenous ligand for TLR4 and initiate destructive immune responses in ischemic brain. Our previous studies showed that ligustilide (LIG) exerted antineuroinflammatory and neuroprotective effects against ischemic insult, but the underlying mechanisms remain unclear. This study investigated whether the TLR4/Prx6 pathway is involved in the protective effect of LIG against postischemic neuroinflammation and brain injury induced by transient middle cerebral artery occlusion (MCAO) in rats. Intraperitoneal LIG administration (20 and 40 mg/kg/day) at reperfusion onset after MCAO resulted in a reduction of brain infarct size and improved neurological outcome over 72 h. LIG-induced neuroprotection was accompanied by improvement of neuropathological alterations, including neuron loss, astrocyte and microglia/macrophage activation, neutrophil and T-Iympliocyte invasion, and regulation of inflammatory mediators expression. Moreover, LIG significantly inhibited the expression and extracellular release of Prx6 and activation of TLR4 signaling, reflected by decreased TLR4 expression, extracellular signal-regulated kinase 1/2 phosphorylation, and transcriptional activity of NF-kappa B and signal transducer and activator of transcription 3 in the ischemic brain. Our results demonstrate that LIG may provide an early and direct neuroprotection by inhibiting TLR4/Prx6 signaling and subsequent immunity and neuroinflammation after cerebral ischemia. These findings support the translational potential of blocking TLR4/Prx6 signaling for the treatment of ischemic stroke. (C) 2014 Elsevier Inc. All rights reserved.
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页码：165 / 175
页数：11
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