The effects of the neonicotinoid imiacloprid on gene expression and DNA methylation in the buff-tailed bumblebee Bombus terrestris

被引:33
作者
Bebane, P. S. A. [1 ]
Hunt, B. J. [2 ]
Pegoraro, M. [1 ]
Jones, A. R. C. [1 ]
Marshall, H. [1 ]
Rosato, E. [1 ]
Mallon, E. B. [1 ]
机构
[1] Univ Leicester, Dept Genet & Genome Biol, Univ Rd, Leicester LE1 7RH, Leics, England
[2] John Moores Univ Liverpool, Sch Nat Sci & Psychol, Liverpool L3 3AF, Merseyside, England
基金
英国生物技术与生命科学研究理事会;
关键词
epigenetics; methylome; RNA-seq; BS-seq; social insects; pesticide; CHRONIC EXPOSURE; SOCIAL INSECTS; GENOME-WIDE; R PACKAGE; BEE; TRANSCRIPTOME; EVOLUTION; METHYLOME; CONSERVATION; EPIGENETICS;
D O I
10.1098/rspb.2019.0718
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Neonicotinoids are effective insecticides used on many important arable and horticultural crops. They are nicotinic acetylcholine receptor agonists which disrupt the function of insect neurons and cause paralysis and death. In addition to direct mortality, there are numerous sublethal effects of low doses of neonicotinoids on bees. We hypothesize that some of these large array of effects could be a consequence of epigenetic changes in bees induced by neonicotinoids. We compared whole methylome (BS-seal) and RNA-seal libraries of the brains of buff-tailed bumblebee Bombus terrestris workers exposed to field-realistic doses of the neonicotinoid imidacloprid to libraries from control workers. We found numerous genes which show differential expression between neonicotinoid-treated bees and control bees, but no differentially methylated cytosines in any context. We found CpG methylation to be focused mainly in exons and associated with highly expressed genes. We discuss the implications of our results for future legislation.
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页数:8
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