Low-Molecular-Mass Penicillin Binding Protein 6b (DacD) Is Required for Efficient GOB-18 Metallo-β-Lactamase Biogenesis in Salmonella enterica and Escherichia coli

被引:8
作者
Brambilla, Luciano
Moran-Barrio, Jorgelina
Viale, Alejandro M. [1 ]
机构
[1] Univ Nacl Rosario, Dept Microbiol, Fac Ciencias Bioquim & Farmaceut, RA-2000 Rosario, Santa Fe, Argentina
关键词
LYTIC TRANSGLYCOSYLASES; ANTIBIOTIC-RESISTANCE; BACTERIAL PERIPLASM; BIOFILM FORMATION; TYPHIMURIUM; GENE; THIOSULFATE; STRESS; PBP5;
D O I
10.1128/AAC.01224-13
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Metallo-beta-lactamases (MBLs) are Zn2+-containing secretory enzymes of clinical relevance, whose final folding and metal ion assembly steps in Gram- negative bacteria occur after secretion of the apo form to the periplasmic space. In the search of periplasmic factors assisting MBL biogenesis, we found that dacD null (Delta dacD) mutants of Salmonella enterica and Escherichia coli expressing the pre-GOB-18 MBL gene from plasmids showed significantly reduced resistance to cefotaxime and concomitant lower accumulation of GOB-18 in the periplasm. This reduced accumulation of GOB-18 resulted from increased accessibility to proteolytic attack in the periplasm, suggesting that the lack of DacD negatively affects the stability of secreted apo MBL forms. Moreover, Delta dacD mutants of S. enterica and E. coli showed an altered ability to develop biofilm growth. DacD is a widely distributed low-molecular-mass (LMM) penicillin binding protein (PBP6b) endowed with low DD-carboxypeptidase activity whose functions are still obscure. Our results indicate roles for DacD in assisting biogenesis of particular secretory macromolecules in Gram-negative bacteria and represent to our knowledge the first reported phenotypes for bacterial mutants lacking this LMM PBP.
引用
收藏
页码:205 / 211
页数:7
相关论文
共 37 条
[1]   CLONING AND CHARACTERIZATION OF A GENE-CLUSTER, PHSBCDEF, NECESSARY FOR THE PRODUCTION OF HYDROGEN-SULFIDE FROM THIOSULFATE BY SALMONELLA-TYPHIMURIUM [J].
ALAMI, N ;
HALLENBECK, PC .
GENE, 1995, 156 (01) :53-57
[2]   IMPROVING THE STABILITY OF A FOREIGN PROTEIN IN THE PERIPLASMIC SPACE OF ESCHERICHIA-COLI [J].
ANBA, J ;
BERNADAC, A ;
LAZDUNSKI, C ;
PAGES, JM .
BIOCHIMIE, 1988, 70 (06) :727-733
[3]  
[Anonymous], 1989, Molecular Cloning: A Laboratory
[4]   Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants:: the Keio collection [J].
Baba, Tomoya ;
Ara, Takeshi ;
Hasegawa, Miki ;
Takai, Yuki ;
Okumura, Yoshiko ;
Baba, Miki ;
Datsenko, Kirill A. ;
Tomita, Masaru ;
Wanner, Barry L. ;
Mori, Hirotada .
MOLECULAR SYSTEMS BIOLOGY, 2006, 2 (1) :2006.0008
[5]   dacD, an Escherichia coli gene encoding a novel penicillin-binding protein (PBP6b) with DD-carboxypeptidase activity [J].
Baquero, MR ;
Bouzon, M ;
Quintela, JC ;
Ayala, JA ;
Moreno, F .
JOURNAL OF BACTERIOLOGY, 1996, 178 (24) :7106-7111
[6]   Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress [J].
Boehm, Alex ;
Steiner, Samuel ;
Zaehringer, Franziska ;
Casanova, Alain ;
Hamburger, Fabienne ;
Ritz, Daniel ;
Keck, Wolfgang ;
Ackermann, Martin ;
Schirmer, Tilman ;
Jenal, Urs .
MOLECULAR MICROBIOLOGY, 2009, 72 (06) :1500-1516
[7]   Importance of antibiotic resistance and resistance mechanisms [J].
Bonomo, Robert A. ;
Rossolini, Gian Maria .
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY, 2008, 6 (05) :549-550
[8]   Moderate deacylation efficiency of DacD explains its ability to partially restore beta-lactam resistance in Escherichia coli PBP5 mutant [J].
Chowdhury, Chiranjit ;
Kar, Debasish ;
Dutta, Mouparna ;
Kumar, Akash ;
Ghosh, Anindya S. .
FEMS MICROBIOLOGY LETTERS, 2012, 337 (01) :73-80
[9]   Metallo-β-lactamases:: Novel weaponry for antibiotic resistance in bacteria [J].
Crowder, Michael W. ;
Spencer, James ;
Vila, Alejandro J. .
ACCOUNTS OF CHEMICAL RESEARCH, 2006, 39 (10) :721-728
[10]   A new heat-shock gene, ppiD, encodes a peptidyl-prolyl isomerase required for folding of outer membrane proteins in Escherichia coli [J].
Dartigalongue, C ;
Raina, S .
EMBO JOURNAL, 1998, 17 (14) :3968-3980