The thiazolidinedione insulin sensitiser, BRL 49653, increases the expression of PPAR-gamma and aP(2) in adipose tissue of high-fat-fed rats

被引：69

作者：

Pearson, SL ^{[1
]}

Cawthorne, MA ^{[1
]}

Clapham, JC ^{[1
]}

Dunmore, SJ ^{[1
]}

Holmes, SD ^{[1
]}

Moore, GBT ^{[1
]}

Smith, SA ^{[1
]}

Tadayyon, M ^{[1
]}

机构：

[1] SMITHKLINE BEECHAM PHARMACEUT,WELWYN GARDEN CIT AL6 9AR,HERTS,ENGLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 229卷 / 03期

关键词：

D O I：

10.1006/bbrc.1996.1876

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of the thiazolidinedione insulin sensitiser BRL 49653 on plasma leptin concentrations and on epididymal fat OB, PPAR-gamma and aP(2) mRNA expression were examined in high-fat-fed and high-carbohydrate-fed adult Wistar rats. Diets were given for 4 weeks, with BRL 49653 (10 mu mol/kg/day) administered by oral gavage for the last 4 days. Treatment with BRL 49653 reduced plasma leptin concentrations in high-fat-fed rats from 2.34 +/- 0.19 (n=9) to 1.42 +/- 0.09 (n=9) ng/ml (p<0.001). Plasma leptin was unaffected by BRL 49653 in the high-carbohydrate-fed rats. There was no difference in OB mRNA expression between high-fat-fed and high-carbohydrate-fed rats, with or without treatment. PPAR-gamma and aP(2) mRNA expression were significantly increased in the high-fat-fed rats treated with BRL 49653 (p<0.01 and p<0.001 respectively), but not in carbohydrate-fed rats. (C) 1996 Academic Press

引用

页码：752 / 757

页数：6

共 23 条

[1] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[2] THE OB GENE AND INSULIN - A RELATIONSHIP LEADING TO CLUES TO THE UNDERSTANDING OF OBESITY [J].

CUSIN, I ;

SAINSBURY, A ;

DOYLE, P ;

ROHNERJEANRENAUD, F ;

JEANRENAUD, B .

DIABETES, 1995, 44 (12) :1467-1470

[3] PATHOGENESIS OF NIDDM - A BALANCED OVERVIEW [J].

DEFRONZO, RA ;

BONADONNA, RC ;

FERRANNINI, E .

DIABETES CARE, 1992, 15 (03) :318-368

[4] LEPTIN LEVELS REFLECT BODY LIPID-CONTENT IN MICE - EVIDENCE FOR DIET-INDUCED RESISTANCE TO LEPTIN ACTION [J].

FREDERICH, RC ;

HAMANN, A ;

ANDERSON, S ;

LOLLMANN, B ;

LOWELL, BB ;

FLIER, JS .

NATURE MEDICINE, 1995, 1 (12) :1311-1314

[5] CHARACTERIZATION OF NEW ORAL ANTIDIABETIC AGENT CS-045 - STUDIES IN KK AND OB OB MICE AND ZUCKER FATTY RATS [J].

FUJIWARA, T ;

YOSHIOKA, S ;

YOSHIOKA, T ;

USHIYAMA, I ;

HORIKOSHI, H .

DIABETES, 1988, 37 (11) :1549-1558

[6] WEIGHT-REDUCING EFFECTS OF THE PLASMA-PROTEIN ENCODED BY THE OBESE GENE [J].

HALAAS, JL ;

GAJIWALA, KS ;

MAFFEI, M ;

COHEN, SL ;

CHAIT, BT ;

RABINOWITZ, D ;

LALLONE, RL ;

BURLEY, SK ;

FRIEDMAN, JM .

SCIENCE, 1995, 269 (5223) :543-546

[7]

IBRAHIMI A, 1994, MOL PHARMACOL, V46, P1070

[8] EFFECT OF NEW ORAL ANTIDIABETIC AGENT CS-045 ON GLUCOSE-TOLERANCE AND INSULIN-SECRETION IN PATIENTS WITH NIDDM [J].

IWAMOTO, Y ;

KUZUYA, T ;

MATSUDA, A ;

AWATA, T ;

KUMAKURA, S ;

INOOKA, G ;

SHIRAISHI, I .

DIABETES CARE, 1991, 14 (11) :1083-1086

[9] CONTROL OF LIPOGENESIS BY DIETARY LINOLEIC-ACID AND ITS INFLUENCE ON THE DEPOSITION OF FAT [J].

JEFFCOAT, R ;

ROBERTS, PA ;

JAMES, AT .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1979, 101 (02) :447-453

[10] SUPPRESSION OF GLUT4 EXPRESSION IN SKELETAL-MUSCLE OF RATS THAT ARE OBESE FROM HIGH-FAT FEEDING BUT NOT FROM HIGH-CARBOHYDRATE FEEDING OR GENETIC OBESITY [J].

KAHN, BB ;

PEDERSEN, O .

ENDOCRINOLOGY, 1993, 132 (01) :13-22

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