Evaluation of the copper(II) reduction assay using bathocuproinedisulfonic acid disodium salt for the total antioxidant capacity assessment: The CUPRAC-BCS assay

被引：118

作者：

Campos, Carlos ^{[1
]}

Guzman, Rodrigo ^{[1
]}

Lopez-Fernandez, Encarnacion ^{[1
]}

Casado, Angela ^{[1
]}

机构：

[1] Spanish Natl Res Council, Biol Res Ctr, Dept Cellular & Mol Physiopathol, E-28040 Madrid, Spain

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 392卷 / 01期

关键词：

CUPRAC-BCS assay; Total antioxidant capacity; Bathocuproinedisulfonic acid disodium salt; TEAC assay; FRAP assay; DPPH assay; ABTS RADICAL-CATION; IN-VITRO; BODY-FLUIDS; HUMAN SERUM; POWER FRAP; PLASMA; IRON; SAMPLE; VIVO;

D O I：

10.1016/j.ab.2009.05.024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

There is heightened interest in determining antioxidant status of individuals in experimental and clinical Studies investigating progression of diseases or diverse aspects of oxidative stress, among others. The aim of this study was to evaluate the copper(II) reduction assay with bathocuproinedisulfonic acid disodium salt as chelating agent (the CUPRAC-BCS assay) for the total antioxidant capacity (TAC) assessment in human plasma and Urine. Samples from 20 individuals were determined with four spectrophotometric assays-CUPRAC-BCS, ferric reducing ability of plasma (FRAP), trolox equivalent antioxidant capacity (TEAC), and 1,1-diphenyl-2-picrylhydrazyl assay (DPPH)-to compare these methods. CUPRAC-BCS was significantly correlated with FRAP and TEAC for plasma and urine samples (r > 0.5, P < 0.05 for all) and with DPPH for urine samples (r = 0.925, P < 0.001) but not with DPPH for plasma samples (r = 0.366, P = 0.112). However, the four methods do not agree given that lines of equality and regression were not matched up. The imprecision of the method is less than 6%. the detection limit is 41.8 mu mol trolox equivalents/L, it is linear up to 2 mM trolox, and ethylenediaminetetraacetic acid dihydrate disodium salt (EDTA) binds to Cu(II), avoiding the formation of Cu(I)-BCS complex. This study shows that CUPRAC-BCS is a simple, fast, inexpensive, and Suitable method for TAC assessment in human urine and heparinized plasma samples. (c) 2009 Elsevier Inc. All rights reserved,

引用

页码：37 / 44

页数：8

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