Cellulose Nanocrystal/Gold Nanoparticle Composite as a Matrix for Enzyme Immobilization

被引：160

作者：

Mahmoud, Khaled A. ^{[1
]}

Male, Keith B. ^{[1
]}

Hrapovic, Sabahudin ^{[1
]}

Luong, John H. T. ^{[1
]}

机构：

[1] Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2, Canada

来源：

ACS APPLIED MATERIALS & INTERFACES | 2009年 / 1卷 / 07期

关键词：

cellulose nanocrystals; gold nanoparticles; enzyme; CGTase; immobilization; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; CYCLODEXTRIN GLUCOSYLTRANSFERASE; GOLD NANOPARTICLES; GLYOXYL-AGAROSE; SUSPENSIONS; SURFACE; GLYCOSYLTRANSFERASE; THERMOANAEROBACTER; STABILIZATION; STABILITY;

D O I：

10.1021/am900331d

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A novel nanocomposite consisting or cellulose nanocrystals (CNCs) functionalized with gold nanoparticles (AuNPs) serving as an excellent support for enzyme immobilization With phenomenally high loading is presented in this work. As testing models, cyclodextrin glycosyl transferase (CGTase) and alcohol oxidase were conjugated on an activated CNC/AuNP matrix. This catalytic platform exhibits significant biocatalytic activity with excellent enzyme stability and without apparent loss of the original activity. the recovered specific activities were similar to 70% and 95% for CGTase and alcohol oxidase, respectively, This novel and inexpensive material is anticipated to extend to other enzymes, enhancing the enzyme loading and activity as well as the stability in both operation and storage.

引用

页码：1383 / 1386

页数：4

共 27 条

[1] Immobilization of Paenibacillus macerans NRRL B-3186 cyclodextrin glucosyltransferase and properties of the immobilized enzyme
Abdel-Naby, MA
[J]. PROCESS BIOCHEMISTRY, 1999, 34 (04) : 399 - 405
[2] Methods and supports for immobilization and stabilization of cyclomaltodextrin glucanotransferase from Thermoanaerobacter
Amud, Ana Elisa
Presa da Silva, Gercio Rodrigo
Tardioli, Paulo Waldir
Faria Soares, Cleide Mara
Moraes, Flavio Faria
Zanin, Gisella Maria
[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2008, 146 (1-3) : 189 - 201
[3] Effect of reaction conditions on the properties and behavior of wood cellulose nanocrystal suspensions
Beck-Candanedo, S
Roman, M
Gray, DG
[J]. BIOMACROMOLECULES, 2005, 6 (02) : 1048 - 1054
[4] COMPARISON OF ORGANIC MONOLAYERS ON POLYCRYSTALLINE GOLD SPONTANEOUSLY ASSEMBLED FROM SOLUTIONS CONTAINING DIALKYL DISULFIDES OR ALKENETHIOLS
BIEBUYCK, HA
BIAN, CD
WHITESIDES, GM
[J]. LANGMUIR, 1994, 10 (06) : 1825 - 1831
[5] PITFALLS OF IMMUNOGOLD LABELING - ANALYSIS BY LIGHT-MICROSCOPY, TRANSMISSION ELECTRON-MICROSCOPY, AND PHOTOELECTRON MICROSCOPY
BIRRELL, GB
HEDBERG, KK
GRIFFITH, OH
[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1987, 35 (08) : 843 - 853
[6] A new step to the mechanism of the enhancement effect of gold nanoparticles on glucose oxidase
Chen, XY
Li, JR
Li, XC
Jiang, L
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 245 (02) : 352 - 355
[7] A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles
Demers, LM
Mirkin, CA
Mucic, RC
Reynolds, RA
Letsinger, RL
Elghanian, R
Viswanadham, G
[J]. ANALYTICAL CHEMISTRY, 2000, 72 (22) : 5535 - 5541
[8] Smooth model cellulose I surfaces from nanocrystal suspensions
Edgar, CD
Gray, DG
[J]. CELLULOSE, 2003, 10 (04) : 299 - 306
[9] POLYMER NANOCOMPOSITES REINFORCED BY CELLULOSE WHISKERS
FAVIER, V
CHANZY, H
CAVAILLE, JY
[J]. MACROMOLECULES, 1995, 28 (18) : 6365 - 6367
[10] Immobilization and stabilization of a cyclodextrin glycosyltransferase by covalent attachment on highly activated glyoxyl-agarose supports
Ferrarotti, Susana Alicia
Bolivar, Juan M.
Mateo, Cesar
Wilson, Lorena
Guisan, Jose M.
Fernandez-Lafuente, Roberto
[J]. BIOTECHNOLOGY PROGRESS, 2006, 22 (04) : 1140 - 1145

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