Pinocembrin attenuates benzo(a)pyrene-induced CYP1A1 expression through multiple pathways: An in vitro and in vivo study

被引:15
作者
Alzahrani, Abdullah M. [1 ]
Rajendran, Peramaiyan [1 ]
机构
[1] King Faisal Univ, Dept Biol Sci, Coll Sci, Al Hasa 31982, Saudi Arabia
关键词
aryl hydrocarbon receptor; benzo(a)pyrene; C57BL; 6; mice; DNA adducts; MAP kinase; pinocembrin; POLYCYCLIC AROMATIC-HYDROCARBONS; AH RECEPTOR; HEPATOCELLULAR-CARCINOMA; CYTOCHROME-P450; 1A1; SIGNALING PATHWAY; OXIDATIVE STRESS; GENE-EXPRESSION; DNA-ADDUCTS; KAPPA-B; ACTIVATION;
D O I
10.1002/jbt.22695
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Benzo(a)pyrene [B(a)P], which is a carcinogen, is a substance most typically known in cigarette smoke and considered as an important intermediary of lung cancer. The enzyme CYP1A1 is crucial for the metabolic conversion of B(a)P into the intermediates that induce carcinogenesis. Stimulation of the aryl hydrocarbon receptor, which is regulated by B(a)P, is thought to induce numerous signaling cascades. Interruption in the mitogen-activated protein kinase (MAPK) pathway causes changes in cellular processes and may alter the AhR pathway. The aim of this investigation is to examine the potential ability of a flavonoid pinocembrin (PCB) to alleviate B(a)P toxicity and analyze the underlying molecular mechanisms. We found that PCB inhibited DNA adduct formation by attenuating CYP1A1 expression through the suppression of the AhR/Src/ERK pathways. PCB mitigated the B(a)P-stimulated DNA damage, inhibited Src and ERK1/2 expression, decreased CYP1A1 expression, and reduced the B(a)P-induced stimulation of NF-kappa B and MAPK signaling in lung epithelial cells. Finally, the activity of CYP1A1 and Src in lung tissues from mice supplemented with PCB was noticeably decreased and lower than that in lung tissues from mice supplemented with B(a)P alone. Collectively, these data suggest that PCB may alleviate the toxic effects of PAHs, which are important environmental pollutants.
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页数:13
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