Fluorescent turn-on determination of the activity of peptidases using peptide templated gold nanoclusters

被引：33
|
作者：
Luo, Junjun [1 ]
Rasooly, Avraham [2 ]
Wang, Liqiang [1 ]
Zeng, Ke [1 ]
Shen, Congcong [1 ]
Qian, Pin [1 ]
Yang, Minghui [1 ]
Qu, Fengli [3 ]
机构：
[1] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China
[2] US FDA, Div Biol, Off Sci & Engn, Silver Spring, MD 20993 USA
[3] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
来源：
MICROCHIMICA ACTA | 2016年 / 183卷 / 02期
基金：
中国国家自然科学基金;
关键词：
beta-Secretases; APP cleaving enzyme; BACE1; beta-amyloid precursor protein; Transmission electron microscopy; Fluorometry; BETA-SECRETASE; SILVER NANOCLUSTERS; INHIBITORS; SITE;
D O I：
10.1007/s00604-015-1683-5
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The fluorescence intensity of gold nanoclusters (AuNCs) is inversely related to the length of a peptide immobilized on its surface. This finding has been exploited to design a turn-on fluorescent method for the determination of the activity of peptidase. The beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) was chosen as a model peptidase. BACE1 cleaves the peptide substrates on AuNCs, and the fluorescence intensity of the AuNCs (at exCitation/emission wavelengths of 320/405 nm) carrying the rest of the cleaved peptide is significantly higher than that of the AuNCs with uncleaved peptide. Transmission electron microscopy revealed a decrease in the size of the AuNCs which is assumed cause fluorescence enhancement. The assay was applied to the determination of BACE1 activity in spiked cell lysates, and recoveries were between 96.9 and 104.0 %.
引用
收藏
页码：605 / 610
页数：6
相关论文
共 50 条
  • [1] Fluorescent turn-on determination of the activity of peptidases using peptide templated gold nanoclusters
    Junjun Luo
    Avraham Rasooly
    Liqiang Wang
    Ke Zeng
    Congcong Shen
    Pin Qian
    Minghui Yang
    Fengli Qu
    Microchimica Acta, 2016, 183 : 605 - 610
  • [2] A fluorescent probe for turn-on sensing of glutathione using red emitting gold nanoclusters
    Varghese, Susan
    Madanan, Anju S.
    Abraham, Merin K.
    Shkhair, Ali Ibrahim
    Indongo, Geneva
    Rajeevan, Greeshma
    Vijila, N. S.
    George, Sony
    CHEMISTRYSELECT, 2024, 9 (08):
  • [3] Turn-on fluorescent nanoprobe for ATP detection based on DNA-templated silver nanoclusters
    Li, Yuxia
    Meng, Zeting
    Liu, Yating
    Zhang, Baozhu
    RSC ADVANCES, 2024, 14 (08) : 5594 - 5599
  • [4] DNA-Templated Silver Nanoclusters for Fluorescence Turn-on Assay of Acetylcholinesterase Activity
    Zhang, Yaodong
    Cai, Yanan
    Qi, Zongli
    Lu, Lu
    Qian, Yunxia
    ANALYTICAL CHEMISTRY, 2013, 85 (17) : 8455 - 8461
  • [5] Glutathione S-transferase templated copper nanoclusters as a fluorescent probe for turn-on sensing of chlorotetracycline
    Wang, Jiaxi
    Chen, Wenting
    Cao, Lei
    Zhou, Mengyan
    Geng, Yongkang
    Liu, Yifei
    Ding, Shushu
    Fu, Ding-Yi
    NANOSCALE ADVANCES, 2024, 6 (02): : 722 - 731
  • [6] A “turn-on” fluorescent sensor for ozone detection in ambient air using protein-directed gold nanoclusters
    Di Wu
    Wenjing Qi
    Chun Liu
    Qing Zhang
    Analytical and Bioanalytical Chemistry, 2017, 409 : 2539 - 2546
  • [7] A "turn-on" fluorescent sensor for ozone detection in ambient air using protein-directed gold nanoclusters
    Wu, Di
    Qi, Wenjing
    Liu, Chun
    Zhang, Qing
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2017, 409 (10) : 2539 - 2546
  • [8] A turn-on fluorescence assay for heparin based on DNA-templated gold nanoclusters via ET
    Ou, LiJuan
    Yang, FaGuo
    Luo, JianXin
    Duan, JiaoJie
    Sun, Aiming
    Chen, LanLan
    Wang, LingYun
    CANADIAN JOURNAL OF CHEMICAL ENGINEERING, 2021, 99 (10): : 2205 - 2210
  • [9] Fluorescence based turn-on strategy for determination of microRNA-155 using DNA-templated copper nanoclusters
    Borghei, Yasaman-Sadat
    Hosseini, Morteza
    Ganjali, Mohammad Reza
    MICROCHIMICA ACTA, 2017, 184 (08) : 2671 - 2677
  • [10] Fluorescence based turn-on strategy for determination of microRNA-155 using DNA-templated copper nanoclusters
    Yasaman-Sadat Borghei
    Morteza Hosseini
    Mohammad Reza Ganjali
    Microchimica Acta, 2017, 184 : 2671 - 2677
12345