Radioprotective effects of a polysaccharide purified from Lactobacillus plantarum-fermented Ishige okamurae against oxidative stress caused by gamma ray-irradiation in zebrafish in vivo model

被引：27

作者：

Lee, WonWoo ^{[1
]}

Kang, Nalae ^{[1
]}

Kim, Eun-A. ^{[1
]}

Yang, Hye-Won ^{[1
]}

Oh, Jae-Young ^{[1
]}

Fernando, Ilekkuttige Priyan Shanura ^{[1
]}

Kim, Kil-Nam ^{[2
]}

Ahn, Ginnae ^{[3
]}

Jeon, You-Jin ^{[1
]}

机构：

[1] Jeju Natl Univ, Dept Marine Life Sci, Jeju 690756, South Korea

[2] Korea Basic Sci Inst, Marine Bio Res Team, Daejeon 690140, South Korea

[3] Chonnam Natl Univ, Coll Fisheries & Ocean Sci, Dept Marine Biofood Sci, Gwangju 500749, South Korea

来源：

JOURNAL OF FUNCTIONAL FOODS | 2017年 / 28卷

基金：

新加坡国家研究基金会;

关键词：

Ishige okamurae; Fermentation; Gamma ray-irradiated zebrafish model; Antioxidant effect; Radioprotective effect; ECKLONIA-CAVA; ANTIOXIDANT ACTIVITY; SULFATED POLYSACCHARIDE; KAPPA-B; ALGINATE; GROWTH; BREVIS; VITRO; PURIFICATION; ACTIVATION;

D O I：

10.1016/j.jff.2016.11.004

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In this study, the effects of a polysaccharide purified from the Celluclast extract of Lactobacillus plantarum-fermented Ishige okamurae (CPFI) against gamma ray-irradiation-induced oxidative stress were investigated in a zebrafish model. CPFI showed an increased extraction yield and high 1,1-dipheny1-2-picrylhydrazyl and hydroxyl radical-scavenging activities. It was further purified using anion-exchange chromatography, and among the six fractions obtained, fraction 2 (AP2), with high glucose and mannose contents, exhibited the highest free radical scavenging activity. AP2 improved zebrafish survival and reduced malformations, such as yolk sac oedema and the occurrence of a bent tail. It also reduced cell death and the production of reactive oxygen species and nitric oxide in the zebrafish. Taken together, these results indicate that the AP2 polysaccharide has radioprotective and antioxidant effects, and is a value-added source of functional food ingredients. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：83 / 89

页数：7

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