OsDSSR1, a novel small peptide, enhances drought tolerance in transgenic rice

被引：31

作者：

Cui, Yanchun ^{[1
]}

Li, Mingjuan ^{[1
]}

Yin, Xuming ^{[1
]}

Song, Shufeng ^{[2
]}

Xu, Guoyun ^{[3
]}

Wang, Manling ^{[1
]}

Li, Chunyong ^{[1
]}

Peng, Can ^{[1
]}

Xia, Xinjie ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China

[2] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China

[3] China Natl Tobacco Corp, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Henan, Peoples R China

来源：

PLANT SCIENCE | 2018年 / 270卷

基金：

中国国家自然科学基金;

关键词：

ABA; Drought stress; Oryza sativa L; OsDSSR1; Small signaling peptide; ZINC-FINGER PROTEIN; AGROBACTERIUM-MEDIATED TRANSFORMATION; INDUCED ANTIOXIDANT DEFENSE; OXIDATIVE STRESS TOLERANCE; SALT-STRESS; ABIOTIC STRESS; PROLINE ACCUMULATION; GENE; ABA; ARABIDOPSIS;

D O I：

10.1016/j.plantsci.2018.02.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Small signaling peptides play important roles in plant development and responses to abiotic and biotic stresses. We have identified a novel small peptide gene in rice, OsDSSR1, which is expressed mainly in the root, stem, node, leaf, and panicle. OsDSSR1 expression is also induced by drought, salinity, ABA, and H2O2 treatment. OsDSSR1 is localized in the nucleus and cytoplasm. Transgenic plants overexpressing OsDSSR1 exhibited enhanced drought stress tolerance and decreased ABA sensitivity as compared to the wild type. Overexpression of OsDSSR1 promoted the accumulation of compatible osmolytes, such as free proline and soluble sugars. OsDSSR1-overexpressing plants displayed enhanced OsSodCc2 and OscAPX expression and superoxide dismutase and ascorbate peroxidase activities under drought stress. RNA-sequencing data revealed that the expression of 72 abiotic stress-responsive genes was significantly altered in homozygous transgenic plants. These stress-responsive candidate genes will aid in expanding our understanding of the mechanisms by which small peptides mediate tolerance in crop species.

引用

页码：85 / 96

页数：12

共 64 条

[1] Peptides as triggers of plant defence [J].

Albert, Markus .

JOURNAL OF EXPERIMENTAL BOTANY, 2013, 64 (17) :5269-5279

[2] Smart Engineering of Genetic Resources for Enhanced Salinity Tolerance in Crop Plants [J].

Arzani, A. ;

Ashraf, M. .

CRITICAL REVIEWS IN PLANT SCIENCES, 2016, 35 (03) :146-189

[3] CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].

BENJAMINI, Y ;

HOCHBERG, Y .

JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300

[4] Why cellular communication during plant reproduction is particularly mediated by CRP signalling [J].

Bircheneder, Susanne ;

Dresselhaus, Thomas .

JOURNAL OF EXPERIMENTAL BOTANY, 2016, 67 (16) :4849-4861

[5] Plant breeding and climate changes [J].

Ceccarelli, S. ;

Grando, S. ;

Maatougui, M. ;

Michael, M. ;

Slash, M. ;

Haghparast, R. ;

Rahmanian, M. ;

Taheri, A. ;

Al-Yassin, A. ;

Benbelkacem, A. ;

Labdi, M. ;

Mimoun, H. ;

Nachit, M. .

JOURNAL OF AGRICULTURAL SCIENCE, 2010, 148 :627-637

[6] A salt-regulated peptide derived from the CAP superfamily protein negatively regulates salt-stress tolerance in Arabidopsis [J].

Chien, Pei-Shan ;

Nam, Hong Gil ;

Chen, Yet-Ran .

JOURNAL OF EXPERIMENTAL BOTANY, 2015, 66 (17) :5301-5313

[7] A large family of genes that share homology with CLAVATA3 [J].

Cock, JM ;

McCormick, S .

PLANT PHYSIOLOGY, 2001, 126 (03) :939-942

[8] OsSGL, a Novel DUF1645 Domain-Containing Protein, Confers Enhanced Drought Tolerance in Transgenic Rice and Arabidopsis [J].

Cui, Yanchun ;

Wang, Manling ;

Zhou, Huina ;

Li, Mingjuan ;

Huang, Lifang ;

Yin, Xuming ;

Zhao, Guoqiang ;

Lin, Fucheng ;

Xia, Xinjie ;

Xu, Guoyun .

FRONTIERS IN PLANT SCIENCE, 2016, 7

[9] Expression of OsMSR3 in Arabidopsis enhances tolerance to cadmium stress [J].

Cui, Yanchun ;

Xu, Guoyun ;

Wang, Manling ;

Yu, Yan ;

Li, Mingjuan ;

da Rocha, Pedro S. C. Ferreira ;

Xia, Xinjie .

PLANT CELL TISSUE AND ORGAN CULTURE, 2013, 113 (02) :331-340

[10] Plant peptides - taking them to the next level [J].

De Coninck, Barbara ;

De Smet, Ive .

JOURNAL OF EXPERIMENTAL BOTANY, 2016, 67 (16) :4791-4795

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