APPROBATION OF PCR-RFLP AND AS-PCR METHODS FOR GENOTYPING CATTLE BY THE DGAT1 GENE

The DGAT1 gene Bos taurus is an economically valuable lipid metabolism gene in cattle, affecting upon milk production and milk quality, the allele polymorphism assessment of which is diagnostically significant. The purpose of this work was to test a number of methods for carrying out PCR-RFLP and AS-PCR for genotyping cattle using allelic variants of the DGAT1 gene and then evaluating allelic polymorphism in the studied animal sample in the context of consistency of the used molecular genetic approaches. Studies have been conducted on a sample of mixed and pure bred Holstein cattle. DNA Extraction from samples of whole canned blood of cattle carried out by the combined alkaline method. Proven methods of genotyping effectively identified the analyzed genotypes, showing consistent with each other reliable results, in accordance with the calculated data of alignments analysis, restriction mapping and simulations of generated PCR and RFLP profiles.