Novel fluorescent protein from Hydnophora rigida possesses green emission

被引:5
作者
Idrees, M. [1 ]
Thangavelu, K.
Sikaroodi, M. [3 ]
Smith, C. [2 ,3 ]
Sivaraman, J. [4 ]
Gillevet, P. M. [3 ]
Bokhari, H. [1 ]
机构
[1] COMSATS Inst Informat Technol, Dept Biosci, Islamabad, Pakistan
[2] US Army, Engn Res & Dev Ctr, Topog Engn Ctr, Alexandria, VA USA
[3] George Mason Univ, Microbiome Anal Ctr, Dept Environm Sci & Policy, Manassas, VA USA
[4] Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore
关键词
Hydnophora rigida; Novel green fluorescent protein; Cloning; Expression; Modeling; MONOMERIC RED; EVOLUTION; MUTANTS; DIVERSITY; ORANGE; MARKER;
D O I
10.1016/j.bbrc.2014.04.042
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fluorescent proteins are a family of proteins capable of producing fluorescence at various specific wavelengths of ultra violet light. We have previously reported the identification and characterization of a novel cyan fluorescent protein (HriCFP) from a reef coral species, Hydnophora rigida. In search of new members of the, diverse family of fluorescent proteins, here we report a new green fluorescent protein (HriGFP) from H. rigida. HriGFP was identified, cloned, expressed in Escherichia coli and purified to homogeneity by metal affinity and size exclusion chromatography. The dynamic light scattering and gel filtration experiments suggested the presence of monomers in solution. The peptide mass fingerprint on the purified protein established the identity of HriGFP. HriGFP had excitation peak at 507 nm and emission peak at 527 nm. HriGFP was similar to HriCFP except the last 16 amino acid sequence at the C-terminal; however, they have shown least similarity with other known fluorescent proteins. Moreover the computational model suggests that HriGFP is a globular protein which consists of 6 alpha-helices and 3 beta-sheets. Taken together our results suggested that HriGFP is a novel naturally occurring fluorescent protein that exists as a monomer in solution. (C) 2014 Elsevier Inc. All right reserved.
引用
收藏
页码:33 / 38
页数:6
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