Novel fluorescent protein from Hydnophora rigida possesses green emission

被引:5
作者
Idrees, M. [1 ]
Thangavelu, K.
Sikaroodi, M. [3 ]
Smith, C. [2 ,3 ]
Sivaraman, J. [4 ]
Gillevet, P. M. [3 ]
Bokhari, H. [1 ]
机构
[1] COMSATS Inst Informat Technol, Dept Biosci, Islamabad, Pakistan
[2] US Army, Engn Res & Dev Ctr, Topog Engn Ctr, Alexandria, VA USA
[3] George Mason Univ, Microbiome Anal Ctr, Dept Environm Sci & Policy, Manassas, VA USA
[4] Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore
关键词
Hydnophora rigida; Novel green fluorescent protein; Cloning; Expression; Modeling; MONOMERIC RED; EVOLUTION; MUTANTS; DIVERSITY; ORANGE; MARKER;
D O I
10.1016/j.bbrc.2014.04.042
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fluorescent proteins are a family of proteins capable of producing fluorescence at various specific wavelengths of ultra violet light. We have previously reported the identification and characterization of a novel cyan fluorescent protein (HriCFP) from a reef coral species, Hydnophora rigida. In search of new members of the, diverse family of fluorescent proteins, here we report a new green fluorescent protein (HriGFP) from H. rigida. HriGFP was identified, cloned, expressed in Escherichia coli and purified to homogeneity by metal affinity and size exclusion chromatography. The dynamic light scattering and gel filtration experiments suggested the presence of monomers in solution. The peptide mass fingerprint on the purified protein established the identity of HriGFP. HriGFP had excitation peak at 507 nm and emission peak at 527 nm. HriGFP was similar to HriCFP except the last 16 amino acid sequence at the C-terminal; however, they have shown least similarity with other known fluorescent proteins. Moreover the computational model suggests that HriGFP is a globular protein which consists of 6 alpha-helices and 3 beta-sheets. Taken together our results suggested that HriGFP is a novel naturally occurring fluorescent protein that exists as a monomer in solution. (C) 2014 Elsevier Inc. All right reserved.
引用
收藏
页码:33 / 38
页数:6
相关论文
共 29 条
  • [1] Genetic Control of Biosynthesis and Transport of Riboflavin and Flavin Nucleotides and Construction of Robust Biotechnological Producers
    Abbas, Charles A.
    Sibirny, Andriy A.
    [J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2011, 75 (02) : 321 - +
  • [2] An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein
    Ando, R
    Hama, H
    Yamamoto-Hino, M
    Mizuno, H
    Miyawaki, A
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) : 12651 - 12656
  • [3] Novel fluorescent protein from Hydnophora rigida possess cyano emission
    Bokhari, H.
    Smith, C.
    Veerendra, K.
    Sivaraman, J.
    Sikaroodi, M.
    Gillevet, P.
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2010, 396 (03) : 631 - 636
  • [4] Cloning of anthozoan fluorescent protein genes
    Carter, RW
    Schmale, MC
    Gibbs, PDL
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 2004, 138 (03): : 259 - 270
  • [5] GREEN FLUORESCENT PROTEIN
    CHALFIE, M
    [J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1995, 62 (04) : 651 - 656
  • [6] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION
    CHALFIE, M
    TU, Y
    EUSKIRCHEN, G
    WARD, WW
    PRASHER, DC
    [J]. SCIENCE, 1994, 263 (5148) : 802 - 805
  • [7] Structural Tuning of the Fluorescent Protein iLOV for Improved Photostability
    Christie, John M.
    Hitomi, Kenichi
    Arvai, Andrew S.
    Hartfield, Kimberly A.
    Mettlen, Marcel
    Pratt, Ashley J.
    Tainer, John A.
    Getzoff, Elizabeth D.
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (26) : 22295 - 22304
  • [8] Photoswitchable cyan fluorescent protein for protein tracking
    Chudakov, DM
    Verkhusha, VV
    Staroverov, DB
    Souslova, EA
    Lukyanov, S
    Lukyanov, KA
    [J]. NATURE BIOTECHNOLOGY, 2004, 22 (11) : 1435 - 1439
  • [9] FACS-optimized mutants of the green fluorescent protein (GFP)
    Cormack, BP
    Valdivia, RH
    Falkow, S
    [J]. GENE, 1996, 173 (01) : 33 - 38
  • [10] Flavin Mononucleotide-Based Fluorescent Reporter Proteins Outperform Green Fluorescent Protein-Like Proteins as Quantitative In Vivo Real-Time Reporters
    Drepper, Thomas
    Huber, Robert
    Heck, Achim
    Circolone, Franco
    Hillmer, Anne-Kathrin
    Buechs, Jochen
    Jaeger, Karl-Erich
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (17) : 5990 - 5994