Electrical detection of DNA via nanoparticles under light-induced assembly

被引:3
作者
Ohashi, Karuna [1 ,2 ,3 ]
Yamamoto, Yasuyuki [1 ,2 ,3 ]
Tamura, Mamoru [1 ,3 ]
Nishimura, Yushi [4 ]
Tokonami, Shiho [2 ,3 ]
Iida, Takuya [1 ,3 ]
机构
[1] Osaka Prefecture Univ, Dept Phys Sci, Grad Sch Sci, Sakai, Osaka 5998570, Japan
[2] Osaka Prefecture Univ, Dept Appl Chem, Grad Sch Sci, Sakai, Osaka 5998570, Japan
[3] Osaka Prefecture Univ, Res Inst Light Induced Accelerat Syst RILACS, Sakai, Osaka 5998570, Japan
[4] Osaka City Univ, Div Mol Mat Sci, Sumiyoshi Ku, Osaka 5588585, Japan
关键词
PLASMONIC NANOPARTICLES; GOLD NANOPARTICLES; DIELECTROPHORESIS; AMPLIFICATION; MANIPULATION; SEQUENCES; TIME;
D O I
10.7567/1347-4065/ab1b67
中图分类号
O59 [应用物理学];
学科分类号
摘要
Rapid, simple, and label-free detection of DNA without fluorescent dyes is desired in the fields of medical diagnosis and food inspection. Electrical measurement using microelectrodes is a promising method for the detection of DNA, whereas it requires multiple pre-treatment processes. In this study, omitting these processes, we demonstrated the possibility of rapid electrical detection of DNA-modified gold nanoparticles and target DNA transported and accumulated between electrodes by photothermal assembly (PTA) under the laser irradiation to a microelectrode. Two types of target DNA molecules with different base sequences were detected within 10 min through the change of electric resistance before and after PTA. The obtained results will be a foundation of a rapid and simple genetic screening method and the detection of various biological nanomaterials based on the electrical measurement mediated by a light-induced assembly. (c) 2019 The Japan Society of Applied Physics
引用
收藏
页数:6
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