Notch signaling stimulates osteogenic differentiation of human bone marrow-derived mesenchymal stem cells

被引：7

作者：

Lu, ZZ ^{[1
]}

Wu, ZZ ^{[1
]}

Zhang, QW ^{[1
]}

Wang, H ^{[1
]}

Jia, XX ^{[1
]}

Duan, HF ^{[1
]}

Wang, LS ^{[1
]}

机构：

[1] Acad Mil Med Sci, Inst Radiat Med, Beijing 100850, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 2004年 / 49卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Notch signaling; mesenchymal stem cells; osteoblasts; dexamethasone; differentiation;

D O I：

10.1360/03wc0617

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Notch signaling is one of the most important pathways mediating cell determination and differentiation. In this study, the roles of Notch signal in the regulation of osteogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) were investigated. The expression of Notch1, Jagged1 and DTX1 detected by reverse transcription polymerase chain reaction (RT-PCR) suggested that Notch signal might exhibit a physiological regulatory role in the differentiation of MSCs. Constitutive expression of the intracellular domain of Notch1 (ICN), the active form of Notch1 protein, can activate Notch signal in cells without ligands' binding. hMSCs were isolated, expanded, and infected with retrovirus carrying green fluorescent protein (GFP) gene or ICN. Overexpression of ICN in hMSCs resulted in enhanced osteogenic differentiation induced by dexamethasone (Dex), which was characterized by an increase of cellular alkaline phosphatase (ALP) activity and calcium deposition. These results indicate that Notch stimulates differentiation of hMSCs into osteoblasts.

引用

页码：815 / 818

页数：4

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