Stereoselective in vitro metabolism of cyproconazole in rat liver microsomes and identification of major metabolites

被引：18

作者：

He, Rujian ^{[1
]}

Fan, Jun ^{[1
]}

Chen, Ran ^{[1
]}

Guo, Dong ^{[1
,2
]}

Zhao, Mengjiu ^{[1
]}

Zhang, Zhifeng ^{[1
]}

Liang, Chuying ^{[1
]}

Chen, Ming ^{[1
]}

Song, Haiyan ^{[1
]}

Zhang, Weiguang ^{[1
]}

机构：

[1] South China Normal Univ, Sch Chem, Guangzhou Key Lab Analyt Chem Biomed, Guangzhou 510006, Peoples R China

[2] Guangzhou Res & Creat Biotechnol Co Ltd, Guangzhou 510663, Peoples R China

来源：

CHEMOSPHERE | 2021年 / 264卷 / 264期

基金：

中国国家自然科学基金;

关键词：

Cyproconazole; Stereoselective metabolism in vitro; Rat liver microsomes; Molecular docking; Metabolic reaction; SPECIES-DIFFERENCES; ENANTIOMERS; MOUSE; SOIL; INHIBITION; FUNGICIDES; METALAXYL; TOXICITY;

D O I：

10.1016/j.chemosphere.2020.128495

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The vast usage of agrochemicals enhances food security globally but may pose challenge to understand the risk assessment to non-target organisms and human beings, and liver microsomes are responsible for metabolism of these agrochemicals in vivo. In this study, stereoselective metabolism of chiral triazole fungicide cyproconazole in rat liver microsomes has been investigated through chiral LC-MS/MS technique. The half-lives of four cyproconazole stereoisomers were different ranging from 95 to 187 min, and (2S, 3R)-cyproconazole preferentially metabolized in rat liver microsomes. In addition, the results from metabolism kinetic study indicated that rat liver microsomes showed the stronger potency to deplete (2S, 3R)-cyproconazole than the others. Then, homology modeling and molecular docking results revealed that the docking energy between (2S, 3R)-cyproconazole and the cytochrome P450 CYP3A1 (-7.46 kcal.mol(-1) ) was higher than the others, meaning that (2S, 3R)-cyproconazole exhibited the strongest binding ability to this enzyme. Moreover, two main metabolites of cyproconazole coming from hydroxylation and dehydration were observed, and possible metabolic reactions of cyproconazole in rat liver microsomes were identified through using an LCQ ion trap mass spectrometer. This kind of systematic metabolic investigation of cyproconazole at chiral level would provide valuable information for ecological and human health risk assessment of chiral pesticides. (C) 2020 Elsevier Ltd. All rights reserved.

引用

页数：8

共 39 条

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