Differentially expressed proteins in gill and skin mucus of Atlantic Salmon (Salmo salar) affected by amoebic gill disease

被引:68
作者
Valdenegro-Vega, Victoria A. [1 ]
Crosbie, Phil [1 ]
Bridle, Andrew [1 ]
Leef, Melanie [1 ]
Wilson, Richard [2 ]
Nowak, Barbara E. [1 ]
机构
[1] Univ Tasmania, NCMCRS, Launceston, Tas 7250, Australia
[2] Univ Tasmania, Cent Sci Lab, Hobart, Tas 7001, Australia
关键词
Amoebic gill disease; Salmo salar; Gill mucus; Skin mucus; Proteomics; NECROSIS-FACTOR-ALPHA; RAINBOW-TROUT; IMMUNE-RESPONSE; GENE-EXPRESSION; NEOPARAMOEBA-PERURANS; SEQUENTIAL PATHOLOGY; ONCORHYNCHUS-MYKISS; PROTEOMIC ANALYSIS; STATISTICAL-MODEL; EPIDERMAL MUCUS;
D O I
10.1016/j.fsi.2014.06.025
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
The external surfaces of fish, such as gill and skin, are covered by mucus, which forms a thin interface between the organism and water. Amoebic gill disease (AGD) is a parasitic condition caused by Neoparamoeba perurans that affects salmonids worldwide. This disease induces excessive mucus production in the gills. The host immune response to AGD is not fully understood, and research tools such as genomics and proteomics could be useful in providing further insight. Gill and skin mucus samples were obtained from Atlantic salmon (Salmo salar) which were infected with N. perurans on four successive occasions. NanoLC tandem mass spectrometry (MS/MS) was used to identify proteins in gill and skin mucus of Atlantic salmon affected by AGD. A total of 186 and 322 non-redundant proteins were identified in gill and skin mucus respectively, based on stringent filtration criteria, and statistics demonstrated that 52 gill and 42 skin mucus proteins were differentially expressed in mucus samples from AGD-affected fish. By generating protein protein interaction networks, some of these proteins formed part of cell to cell signalling and inflammation pathways, such as C-reactive protein, apolipoprotein 1, granulin, cathepsin, angiogenin-1. In addition to proteins that were entirely novel in the context in the host response to N. perurans, our results have confirmed the presence of protein markers in mucus that have been previously predicted on the basis of modified mRNA expression, such as anterior gradient-2 protein, annexin A-1 and complement C3 factor. This first proteomic analysis of AGO-affected salmon provides new information on the effect of AGD on protein composition of gill and skin mucus. Future research should focus on better understanding of the role these components play in the response against infection with N. perurans. (c) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:69 / 77
页数:9
相关论文
共 84 条
[1]   Sequential pathology after initial freshwater bath treatment for amoebic gill disease in cultured Atlantic salmon, Salmo salar L. [J].
Adams, MB ;
Nowak, BF .
JOURNAL OF FISH DISEASES, 2004, 27 (03) :163-173
[2]   Amoebic gill disease:: sequential pathology in cultured Atlantic salmon, Salmo salar L. [J].
Adams, MB ;
Nowak, BF .
JOURNAL OF FISH DISEASES, 2003, 26 (10) :601-614
[3]   Distribution and structure of lesions in the gills of Atlantic salmon, Salmo salar L., affected with amoebic gill disease [J].
Adams, MB ;
Nowak, BF .
JOURNAL OF FISH DISEASES, 2001, 24 (09) :535-542
[4]   Impact of Lactose Starvation on the Physiology of Lactobacillus casei GCRL163 in the Presence or Absence of Tween 80 [J].
Al-Naseri, Ali ;
Bowman, John P. ;
Wilson, Richard ;
Nilsson, Rolf E. ;
Britz, Margaret L. .
JOURNAL OF PROTEOME RESEARCH, 2013, 12 (11) :5313-5322
[5]   Transgelin: An actin-binding protein and tumour suppressor [J].
Assinder, Stephen J. ;
Stanton, Jo-Ann L. ;
Prasad, Priya D. .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2009, 41 (03) :482-486
[6]   The acute phase response and innate immunity of fish [J].
Bayne, CJ ;
Gerwick, L .
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2001, 25 (8-9) :725-743
[7]   Major Vault Protein Regulates Class A Scavenger Receptor-mediated Tumor Necrosis Factor-α Synthesis and Apoptosis in Macrophages [J].
Ben, Jingjing ;
Zhang, Yan ;
Zhou, Rongmei ;
Zhang, Haiyang ;
Zhu, Xudong ;
Li, Xiaoyu ;
Zhang, Hanwen ;
Li, Nan ;
Zhou, Xiaodan ;
Bai, Hui ;
Yang, Qing ;
Li, Donghai ;
Xu, Yong ;
Chen, Qi .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2013, 288 (27) :20076-20084
[8]   C-reactive protein [J].
Black, S ;
Kushner, I ;
Samols, D .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (47) :48487-48490
[9]   The serum proteome of Atlantic salmon, Salmo salar, during pancreas disease (PD) following infection with salmonid alphaviru.s subtype 3 (SAV3) [J].
Braceland, M. ;
Bickerdike, R. ;
Tinsley, J. ;
Cockerill, D. ;
Mcloughlin, M. F. ;
Graham, D. A. ;
Burchmore, R. J. ;
Weir, W. ;
Wallace, C. ;
Eckersall, P. D. .
JOURNAL OF PROTEOMICS, 2013, 94 :423-436
[10]   Quantitation of immune response gene expression and cellular localisation of interleukin-1β mRNA in Atlantic salmon, Salmo salar L., affected by amoebic gill disease (AGD) [J].
Bridle, Andrew R. ;
Morrison, Richard N. ;
Cunningham, Pauline M. Cupit ;
Nowak, Barbara F. .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2006, 114 (1-2) :121-134