Surface plasmon resonance biosensors for simultaneous monitoring of amyloid-beta oligomers and fibrils and screening of select modulators

被引：41

作者：

Yi, Xinyao ^{[1
,2
]}

Feng, Chengting ^{[1
]}

Hu, Shengqiang ^{[1
]}

Li, Hengfeng ^{[2
]}

Wang, Jianxiu ^{[1
]}

机构：

[1] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China

[2] Cent S Univ, Sch Mat Sci & Engn, Changsha 410083, Hunan, Peoples R China

来源：

ANALYST | 2016年 / 141卷 / 01期

基金：

中国国家自然科学基金;

关键词：

ALZHEIMER A-BETA; METHYLENE-BLUE; IN-VIVO; DISEASE; AGGREGATION; INHIBITORS; ANTIBODIES; PEPTIDE; COMMON; POTENTIATION;

D O I：

10.1039/c5an01864a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Oligomeric amyloid-beta (A beta) peptides are considered as the most toxic species in Alzheimer's disease (AD). Monitoring of the A beta aggregation profiles is critical for elucidating the oligomer toxicity and may serve as a therapeutic target for AD. By immobilizing the capture antibodies of A11 and OC that are specific to the oligomers and fibrils, respectively, in separate fluidic channels, a novel surface plasmon resonance (SPR) biosensor was designed for monitoring the oligomeric and fibrillar species of A beta(1-42) simultaneously. The influence of curcumin, Cu2+ and methylene blue on the amount of toxic oligomers and fibrils was evaluated. The half maximal inhibitory concentration (IC50) of curcumin and methylene blue was determined. The formation of A beta fibrils was also validated by the thioflavin T (ThT) fluorescence assay. The results demonstrate the utility of SPR as an analytical tool for rapid and comprehensive monitoring of A beta aggregation and screening of Aa modulators.

引用

页码：331 / 336

页数：6

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