Antiproliferative Effect of 1α,25-dihydroxyvitamin D3 Involves Upregulation of Cyclin-Dependent Kinase Inhibitor p21 in Human Pancreatic Cancer Cells

被引：16
|
作者：
Kanemaru, Mikio [1 ]
Maehara, Naoki [1 ]
Chijiiwa, Kazuo [1 ]
机构：
[1] Miyazaki Univ, Sch Med, Dept Surg Oncol & Regulat Organ Funct, Kiyotake, Miyazaki 8891692, Japan
来源：
HEPATO-GASTROENTEROLOGY | 2013年 / 60卷 / 125期
关键词：
1; alpha; 25-dihydroxyvitamin D3; VDR; p21; Pancreatic cancer; Proliferation; VITAMIN-D ANALOGS; PROSTATE-CANCER; ELEVATED EXPRESSION; GEMCITABINE; D-3; MORTALITY; LINE; 1,25-DIHYDROXYVITAMIN-D3; DIFFERENTIATION; 1,25(OH)(2)D-3;
D O I：
10.5754/hge11073
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
Background/Aims: The aim of this study was to investigate the effect of 1 alpha,25-dihydroxyvitamin D3 on proliferation of human pancreatic cancer cell lines and to identify related cell cycle regulatory proteins with antiproliferative effects. Methodology: Human pancreatic cancer cell lines SUIT-2 and its four sublines, and Panc-1, AsPC-1, and MiaPaCa-2 were treated with1 alpha,25-dihydroxyvitamin D3. The number of cells was measured by the MTT method, and the cell cycle regulatory proteins were then analyzed by Western blotting. Results: Eight human pancreatic cancer cell lines expressed vitamin D receptor (VDR) mRNA. 1 alpha,25-dihydroxyvitamin D3 inhibited proliferation of SUIT-2 and its sublines. We found p21 to be upregulated after 24 hours in S2-028, the cell line in which proliferation was most inhibited by 1 alpha,25-dihydroxyvitamin D3. Conclusions: 1 alpha,25-dihydroxyvitamin D3 inhibited proliferation of pancreatic cancer cells and is involved in the upregulation of cyclin-dependent kinase inhibitor p21.
引用
收藏
页码：1199 / 1205
页数：7
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